How to do this technique using culture procedures? - (Apr/17/2008 )

I want to study the effect of a certain drug on the blood and some organs of the mouse.

how can i benefit from the culture method in order to stydy it in vitro????

i need information about how to do culture for blood or any other tissues????

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most organs, or let´s say tissues have model cells to study in vitro; you may start with primary cell culture; oncogenic cell lines are often used cells as they are, in most cases, easy to handle and immortal

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i want mor detail information if you could help me in this topic as i'm new in this field.

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you may offer the organs/tissues/cells you really want to analyze, so that we can open discussion in more detail; I regret that I cannot recommend for blood cells; the only one I use are Jurkat cells (T-cells)

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thank you so much for ur help
my problem is that i study the effect of some drugs on the mutation of some genes within rat genome. but i study this effects in vivo. and get the DNA from blood lymphocytes.
now i want to study those effects in vitro.
so what could i do now??
doing culture for lymphocytes??
then what????

best regards.

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