Maize tissue culture - (Jun/11/2006 )

Hi,

I am new in tissue culture. I will going to do the maize tissue culture soon but I don't have the protocol for maize tissue culture. I will going to do the research on maize Agrobacterium transformation. What media I should use for callus culture initiation from maize mature seed?Anyone can give your helping hand? Thank you!

Cheers,
Wayne

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Hi Wayne!
I'd like to help you, but unfortunately I don't have any experience in maize culture.
May I post a question for you instead of a reply?
Why did you choose A. tumefaciens transformation strategy? (I have some experience in that field, so if you have some additional question...) I mean: maize is a monocot, isn't it? I thought that A. tumefaciens method is hardly appliable to monocots...am I totally wrong?????
I hope you will share your view!
Thank you!

ILA

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yes ila is right try biolistic method for maiz.

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HI GUYS!

Today I found this paper!
That's about Agrob. transformation of maize callus...so, at the end it seems that I could have been totally wrong ...
what do you think? Is Agrob. a suitable agent to transform monocots???

Thanks for sharing your ideas!!!

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Hi ila,

Thanks for ur help. What i know there are many successful cases transform used agro. My lab done a lots of transformation using agro for oil palm n rice (both monocot). So i dont think is a problem if use agro to transform monocot.

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Hi ila,

Thanks for ur help. What i know there are many successful cases transform used agro. My lab done a lots of transformation using agro for oil palm n rice (both monocot). So i dont think is a problem if use agro to transform monocot.

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