small intensely fluorescent cells - how to culture them? (Sep/07/2007 )
Hey guys, I am starting out in a lab and I wanted to see if anyone on this forum has any experience culturing Small intensely fluorescent cells. I've been digging through old literature and trying to follow the procedures. I haven't had any success so far. Any thoughts?
I am not exactly sure what you do mean by small intensely fluorescent cells. Stable transfected cells with a fluorescent protein, or cells loaded with a dye?
Small intensely fluorescent cells are the name of a type of cell located in many parts of the body including sympathetic ganglia. they are a neural crest derivative that shares a common pregenitor cell along with sympathetic neurons and chromaffin cells.
i would do regular dissociation with papain and standard culture methods for neuronal cells. it might be of use to pass freshly dissociated cells through the cell sorter to select for the fluorescent cells.
Some groups dissect out the sympathetic ganglia for analysis in diabetes model in rats/mice. The changes in the ganglia reflect aging and neuroaxonal dystrophy.