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primary culture - measuring transcription - mRNA expression in primary culture (Aug/23/2005 )

I'm doing an assay in my primary culture cells. I wonder how long should I stimulate my cells so that I could detect transcribtion of my target gene.


it depends on a lot of paramaters which are specific for each gene...
better is to make some kinetics
stimulate your cells for 4 and 16 hours for beginning and you will advise after first results in the next experiment wink.gif