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Electrophoresis Related Discussions
  1. agarose gel electrophoresis of RNA - (reply: 1)
  2. EtBr in agarose gel and running buffer - (reply: 3)
  3. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
  4. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
  5. Agarose gel bands gone (for gel extraction) - (reply: 2)
  6. About Gel supershift - (reply: 1)
  7. siRNA Agarose gel electrophoresis - Protocol - (reply: 1)
  8. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  9. cDNA test on 1% agarose gel - (reply: 3)
  10. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 1)
  11. Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
  12. Help with bad electrophoresis run. - (reply: 3)
  13. Quantify DNA in gel electrophoresis bands - (reply: 7)
  14. How to make gradient gel (10-20%), how to control the speed of flow? - (reply: 4)
  15. Low yield PCR product after gel purification - (reply: 7)
  16. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
  17. Straight bands on DNA gel - (reply: 3)
  18. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  19. Electrophoresis combs - Mini-plus vs Midi - (reply: 7)
  20. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  21. reason of vertical streaks on gels - (reply: 1)
  22. Reused glass bottles to make ethidium bromide gels - (reply: 1)
  23. Just built a gel chamber, blue LED transilluminator, ect.. selling - (reply: 2)
  24. PCR product running on agarose gel - (reply: 32)
  25. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  26. Blue fluorescent color of agarose gel in UV - (reply: 7)
  27. gel shift assay by chemilumniscence. - (reply: 1)
  28. Electrophoresis problem , please help pals ! - (reply: 8)
  29. Western blot question- High % Gel/Low MW Protein - (reply: 7)
  30. too much DNA on agarose gel - (reply: 9)
  31. polyacrylamide RNA gel - (reply: 4)
  32. isoelctric gels hard to stain? - (reply: 2)
  33. protein sample floating out of the tricine gel - (reply: 3)
  34. Protein Purification from Polyacrylamide Gels - (reply: 3)
  35. bands position in agarose gel - (reply: 4)
  36. Protein degradation on 2D gels - (reply: 2)
  37. SDS PAGE gel storing - (reply: 3)
  38. White bands on Cy3 gels - (reply: 1)
  39. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
  40. mini printer for printing gel images to insert into lab book - (reply: 13)
  41. SDS PAGE gels - (reply: 5)
  42. Premixing gel solutions - (reply: 2)
  43. Odd Bands on Gel - (reply: 3)
  44. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
  45. qPCR: band in gel, but super high Cq? - (reply: 10)
  46. How to avoid gel breakage after electrophoresis - (reply: 5)
  47. A good UV transilluminator (electrophoresis) - (reply: 4)
  48. Agarose gell electrophoresis - (reply: 1)
  49. Please look at my gel picture, poor EthBr staining - (reply: 2)
  50. Good loading control for native gels? - (reply: 1)
  51. Pattern/cloud appearing on agarose gel - (reply: 15)
  52. no product on gel after restriction digestion - (reply: 3)
  53. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
  54. TAU gel electrophoresis of histones - (reply: 4)
  55. Problems with Electrophoresis of RNA - (reply: 3)
  56. Problems with having to run the gels for so long - (reply: 14)
  57. Ethidium Bromide Agarose Gel Hazard - (reply: 6)
  58. Single Plasmid Gel Band - (reply: 1)
  59. Buffer in Gel - (reply: 6)
  60. how do GST-tags affect gel filtration results? - (reply: 5)
  61. problems with recovery of dna from gel and ligation - (reply: 5)
  62. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
  63. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  64. QIAquick Gel Extraction - (reply: 2)
  65. agarose gel electrophoresis - (reply: 5)
  66. Uneven bands denaturing agarose - (reply: 2)
  67. Acid urea gel electrophoresis (AUGE) - (reply: 7)
  68. storing the loaded gel before running it - (reply: 7)
  69. What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely - (reply: 7)
  70. Smear on agarose gel from QIAGEN extracted plant samples - (reply: 1)
  71. E gels and the E gel opener - (reply: 1)
  72. SYBR gold post-stain makes blurry gels - (reply: 1)
  73. Dyes for RNA in Urea-polyacrylamide gel - (reply: 1)
  74. Coomassie stain forzen and thawed gel? - (reply: 1)
  75. PCR band moves lower than its size on agarose gel - (reply: 3)
  76. IEF gel detaching during run - (reply: 7)
  77. Agarose gel electrophoresis of RNA - (reply: 5)
  78. What causes surface fluorescence on agarose gels? - (reply: 6)
  79. Problems with PAGE gels - (reply: 1)
  80. oligo continually appears less on gel compared to other same load - (reply: 2)
  81. can coommassie stained gel be western blot - (reply: 5)
  82. carpet in gels for PCR products - (reply: 2)
  83. What % native urea gel do I use for Fab and F(ab')2? - (reply: 2)
  84. protein extraction from GEL - (reply: 5)
  85. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  86. Odd gel run following PCR - (reply: 28)
  87. Problem with visibility on third comb in gels - (reply: 4)
  88. how much time for prepared gel to keep bfore loading? - (reply: 5)
  89. Transfer of proteins from gel to PVDF for short period - (reply: 3)
  90. Product help: Inexpensive Agarose Gel - (reply: 1)
  91. what is your DNA concentration after gel extraction? - (reply: 5)
  92. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  93. Vertical Vs horizontal electrophoresis - (reply: 3)
  94. RNase h electrophoresis - (reply: 1)
  95. RNA degradation problem, only one band on the gel - (reply: 3)
  96. rtPCR working but the elongated product wont gel! - (reply: 1)
  97. Using 4-year old precast gels - (reply: 4)
  98. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  99. Using out-dated equipment to image agarose gel samples-help - (reply: 1)
  100. Second round of PCR after gel extraction fails miserably - (reply: 3)
  101. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
  102. No bands appearing in DNA gel electrophoresis - (reply: 1)
  103. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
  104. What percentage should I use for a stacking gel? - (reply: 1)
  105. What percentage gel should I make? - (reply: 1)
  106. White big cloud in agar gel electrophoresis - (reply: 13)
  107. Signal stuck on top of seperating gel - (reply: 1)
  108. SDS-PAGE gel and running buffer - (reply: 3)
  109. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  110. Agarose Gel - (reply: 1)
  111. Denaturing agent Acrylamide-bisacruylamide gel - (reply: 6)
  112. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
  113. Vertical agarose gel electrophoresis for DNA - (reply: 1)
  114. Running Primers on Agarose Gel - (reply: 6)
  115. strange bands shape agarose gel - (reply: 2)
  116. Estimating size of PCR products in a agarose gel - (reply: 4)
  117. Ladders not resolving on agarose gel - (reply: 6)
  118. IEF migration to middle of gel, no separation - (reply: 2)
  119. how to dissolve Polyacrylamide gel? - (reply: 8)
  120. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
  121. Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
  122. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
  123. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  124. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
  125. restaining of coommassie destained gel - (reply: 1)
  126. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  127. how to make nondenaturing gradient gel - (reply: 2)
  128. Pulsed Field Gel Electrophoresis - (reply: 4)
  129. Sphere staining protocol (agarose) - (reply: 3)
  130. How to interpret this agarose gel? - (reply: 6)
  131. Ligation gel picture interpretation - (reply: 4)
  132. Is there an optimum % agarose or... "more is better"? - (reply: 2)
  133. Western gels running super slow! - (reply: 3)
  134. How to use the OWL Gel Casting System? - (reply: 2)
  135. RNA electrophoresis - (reply: 4)
  136. DNA ladder running faster than samples during agarose gel electrophoresis - (reply: 1)
  137. Troubles with casting Gradient Gels - (reply: 2)
  138. Polyclonal antibody production using SDS-PAGE gel fragment - (reply: 3)
  139. total RNA Extraction gel control - (reply: 1)
  140. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
  141. PAGE gel recipes - (reply: 1)
  142. SB buffer and agarose: Fragments less than 100 bp - (reply: 1)
  143. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  144. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
  145. PEG 8000 precipitation/ gel electrophoresis - (reply: 5)
  146. Software to construct a cladogram from a gel - (reply: 2)
  147. Empty agarose gel after electrophoresis - (reply: 1)
  148. Bright bands of PCR products stick in gel wells - (reply: 1)
  149. DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
  150. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  151. polyacrylamide gel problem....slipping - (reply: 5)
  152. Anoyone knows a good reliable gel documentation system? - (reply: 4)
  153. how to seperate double-digestion products on agarose gel - (reply: 14)
  154. Samples leaking out of wells in gel - (reply: 2)
  155. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  156. syto9 for DNA gel electrophoresis - (reply: 1)
  157. I can't see anything in my gel. I need help - (reply: 1)
  158. Help with DNA acrylamide gel - leaking and errors during gel running - (reply: 4)
  159. Bradford reagent for staining gels? - (reply: 1)
  160. wrong size in agarose gel, right size in sequencing - (reply: 7)
  161. 1D analysis electrophoresis software for automated labelling - (reply: 3)
  162. Weird DNA electrophoresis result - (reply: 4)
  163. DNA migrate differently in agarose vs PAGE gel - (reply: 4)
  164. RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
  165. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  166. poor seal between stacking a seperating gels - (reply: 4)
  167. discarding acrylamide gels?? - (reply: 2)
  168. Agarose - (reply: 7)
  169. IEF technique utilizing Multiphor II Electrophoresis System - I am totally a newbie (reply: 9)
  170. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
  171. Problem with protein resolving in electrophoresis - (reply: 7)
  172. RNA degradation during electrophoresis?? - (reply: 2)
  173. gel question - (reply: 3)
  174. electrophoresis rflp help - (reply: 1)
  175. Stacking gel for DNA PAGE - (reply: 2)
  176. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  177. Bioanalyzer and RNA gel - (reply: 2)
  178. EMSA + WB - Transferring 6% native gel (reply: 1)
  179. SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
  180. storing SDS-PAGE gels for W.Blot - (reply: 7)
  181. acrylamide gel for low molecular weight protein - (reply: 2)
  182. how many samples should i prepare? - about 2 dimensional electrophoresis (reply: 2)
  183. The Best Gel Extraction Kits - The Best Gel Extraction Kits (reply: 9)
  184. agarose gel has melten - (reply: 4)
  185. Dna after gel extraction, single or double stranded? - (reply: 1)
  186. Checking RNA on gel after DIG labeling - (reply: 1)
  187. Gels 'blobby' - (reply: 2)
  188. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
  189. Gel filtration chromatography - for fractionation of lysate (reply: 4)
  190. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  191. Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
  192. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
  193. SDS-PAGE gel wont separate small proteins - (reply: 5)
  194. SDS Acrylamide Gel Equipement - Need to purchase best and cheapest new equipment (reply: 3)
  195. PCR Gel nonspecific band - (reply: 13)
  196. Single-digested vector shifts downward on gel - (reply: 1)
  197. Funny DNA bands on Agarose gels - (reply: 6)
  198. leaving gel in buffer ! - (reply: 3)
  199. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
  200. 2d gel technique - (reply: 2)
  201. Gel Extraction using buffer QG then phenol/chl - Can I? (reply: 1)
  202. Tricine-SDS gels blotting problems - (reply: 4)
  203. native PAGE gel - (reply: 2)
  204. Method to dissolve agarose gel? - (reply: 2)
  205. Problem loading DNA / agarose gel - (reply: 11)
  206. Which buffer do you use for gel electrophoresis? - (reply: 9)
  207. Agarose Gel Disposal - (reply: 13)
  208. Problem Viewing bands in Agarose Gel - (reply: 6)
  209. Agarose gel loading sample pops out - Sample pops out when loading gel (reply: 3)
  210. agarose gel electrophoresis - (reply: 3)
  211. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  212. No bands on agarose gel after restriction digestion. - (reply: 10)
  213. Gel filtration Chromatography - (reply: 1)
  214. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
  215. Stacking Gel Polymerization - (reply: 4)
  216. not "that" 2D gel - (reply: 2)
  217. Extra band on gel after miniprep - (reply: 6)
  218. 3 distinct bands of gDNA on gel - (reply: 2)
  219. Dry protein gels without cracking - (reply: 5)
  220. power supply electrophoresis - (reply: 11)
  221. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  222. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
  223. resolving gel pourcentage - (reply: 3)
  224. Total RNA only produce 1 band in gel - (reply: 2)
  225. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 3)
  226. How to modify electrophoresis chamber - (reply: 3)
  227. NuPAGE Gel get smaller after staining o/n - (reply: 1)
  228. RNA in native gel - (reply: 3)
  229. gel documentation system broken - any ideas how to fix? (reply: 2)
  230. How to reduce electrophoresis times - Impatient me needs faster results (reply: 16)
  231. question about plating cells - gel or no gel? (reply: 2)
  232. DNA, Gel - Possible reasons for DNA Gel Smear (reply: 12)
  233. TBE/Agarose Gel Concentrations - (reply: 3)
  234. Agarose gel to separate btween 4800bp and 4850bp - (reply: 3)
  235. Electrophoretic Mobility Shift Assay - no free probes shown in samples (reply: 3)
  236. Denaturing gel electrophoresis for qPCR - (reply: 3)
  237. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  238. Lithium electrophoresis buffer - Why does it not work? (reply: 2)
  239. Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
  240. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
  241. PCR, using gel extracted band as a template - (reply: 4)
  242. Which gel concentration - (reply: 4)
  243. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  244. Combining forward and reverse primer gives different size on gel - (reply: 2)
  245. Agarose Gel Electrophoreses - What makes it fluoresce? - (reply: 3)
  246. TBE Acrylamide/Formamide gels running slowly - (reply: 4)
  247. microinjection of zebrafish - the agarose plate - where to buy the mould for the grooves (reply: 1)
  248. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
  249. making gel electrophoresis - (reply: 1)
  250. Running a western with cell media in gels? - (reply: 11)
  251. Gel electrophoresis - (reply: 4)
  252. running problems SDS-PAGE gel (zymography) - (reply: 4)
  253. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  254. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  255. Estimation of DNA Quantity on an Agarose Gel - (reply: 5)
  256. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  257. Electrophoresis: sometimes mA=0 - (reply: 3)
  258. low melt agarose - (reply: 1)
  259. Agarose Gel electrophoresis - (reply: 2)
  260. Problems with qiaquick gel purification kit - cloning (reply: 10)
  261. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
  262. strange problem in PAGE gel - (reply: 12)
  263. Anomalous asymmetrical gel - (reply: 1)
  264. Noisy fluorescent gels - (reply: 1)
  265. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  266. got smear on the PCR gel !!! - (reply: 4)
  267. MaestroGen UltraSlim LED illuminator - for DNA gel (reply: 1)
  268. Gel Electrophoresis Question - (reply: 12)
  269. Backwards electrophoresis electrodes - Can you dig it? (reply: 1)
  270. resolving and stucking gel solutions - (reply: 2)
  271. SDS PAGE - Bubble inside the Gel (reply: 5)
  272. serum separation: transparent gel prevents serum collection! - (reply: 2)
  273. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  274. checking sonication using chelex and agarose gel - (reply: 10)
  275. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
  276. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  277. uncut vector contamination in gel purified band - (reply: 2)
  278. Agarose gel electrophresis - (reply: 3)
  279. Genomic DNA electrophoresis? - (reply: 1)
  280. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
  281. polyacrylamide gel storage - (reply: 3)
  282. RNA degradation in collagen gels - (reply: 1)
  283. making gel in advance - to save time (reply: 7)
  284. Agarose gel bands smear/ drag problem - (reply: 4)
  285. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  286. gel does not polymerize - (reply: 12)
  287. Gel Storage for PAGE - Advance casting of polyacrylamide gels (reply: 2)
  288. How many volts/milliamps to run gel at? - (reply: 2)
  289. EMSA gels - (reply: 2)
  290. 6.7%Polyacrylamide/50% Urea gel - (reply: 1)
  291. Migration Distance on a Gel - (reply: 2)
  292. Varied resolution in the same gel - (reply: 2)
  293. Large DNA extraction from an agarose gel - (reply: 8)
  294. Strange SDS gel after Western Blotting - (reply: 1)
  295. Lost protein after gel filtration - Gel Filtration (reply: 6)
  296. Electrophoresis problems - (reply: 6)
  297. how to do gel documentation - (reply: 2)
  298. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
  299. Does agarose go bad? - (reply: 14)
  300. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
  301. Problem- wierd 2D gel image - (reply: 5)
  302. Agarose gel electrophoresis question - (reply: 1)
  303. H3 in tris tricine gels - (reply: 2)
  304. Microcentrifuge Gel Filtration Question - (reply: 4)
  305. SB Buffer and Page Electrophoresis - (reply: 3)
  306. What causes this offset band on RIPA gel? - (reply: 4)
  307. Running real time pcr product on gel?.. - (reply: 4)
  308. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
  309. DNA quantification after gel purification - (reply: 6)
  310. Please help me to choose right Gel imager!!! - (reply: 2)
  311. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  312. Breast Feeding and Ethiduim Bromide - Can you make agarose gels? (reply: 10)
  313. Western blot problems - No or vey little transfer, expanding gel in transfer (reply: 5)
  314. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  315. Problem with gel extraction of DNA. - (reply: 1)
  316. GHOST in Electrophoresis - (reply: 18)
  317. Akrylamide Gels - (reply: 2)
  318. gel filtration.. - (reply: 1)
  319. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
  320. Making gels in advance - (reply: 2)
  321. No protein spots in 2D gel - Silver staining! - (reply: 17)
  322. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
  323. Probelm with gel filtration - (reply: 4)
  324. 3% NuSieve gel electrophoresis - Why 1kb ladder doesn't move well? (reply: 3)
  325. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  326. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  327. Agarose / SDS Page - (reply: 2)
  328. GEL SMEAR - (reply: 4)
  329. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
  330. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  331. problem about DNA electrophoresis - the DNAs become two layers in the gel (reply: 4)
  332. No SDS in gels - (reply: 12)
  333. densidometric analysis with scans - no gels available!!! (reply: 4)
  334. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  335. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  336. Extraction of DNA smaller than 100 bp from Agarose Gel - (reply: 6)
  337. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  338. How to view 75 bp DNA in gel - (reply: 4)
  339. gel documentation sysytem - (reply: 7)
  340. Gel Running Crooked?? Please help.. - (reply: 7)
  341. peptide extraction tricine gel - (reply: 1)
  342. the ChIP agarose gel results - Should the input always be more than the IP one? (reply: 2)
  343. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
  344. DNA damage in gel - (reply: 8)
  345. Software for labeling gel picture - (reply: 1)
  346. Can i use agarose gel to seperate 80bp - (reply: 2)
  347. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  348. Re-using unloaded agarose gel - (reply: 6)
  349. PCR product appear two close band in my gel - (reply: 15)
  350. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  351. Destaining a coomassie sttained gel - (reply: 14)
  352. 5% agarose - (reply: 4)
  353. Too much failure in electrophoresis, am I doing the gel correctly? - (reply: 10)
  354. Desperate! Loss of basic proteins on 2D gels - (reply: 5)
  355. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  356. Sodium borate buffer gel problems - (reply: 5)
  357. help with gel shift assay - (reply: 1)
  358. Run PCR amplifications in agarose gels - (reply: 3)
  359. Aggregated samples at the top of SDS-PAGE gel - (reply: 3)
  360. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  361. Silver stained 2-d gel BLOB!!! - (reply: 1)
  362. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
  363. Problems with small peptide electrophoresis - (reply: 2)
  364. EMSA gel problem - (reply: 2)
  365. Harvesting cells off of collagen gels - (reply: 1)
  366. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  367. Simple gel question - (reply: 7)
  368. DNA agarose gel electrophoresis - sum1 can teach me interpret this gel image.. (reply: 3)
  369. 2 d gel electrophoresis consumable - any brand? (reply: 1)
  370. Pulse field gel electrophoresis - (reply: 2)
  371. PCR bands on gel electrophoresis - (reply: 5)
  372. to keep sds-pa gels - (reply: 2)
  373. hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
  374. Hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - Well,actually I want to make some homemade agarose beads,which haven't (reply: 5)
  375. Smearing on gel in PCR products - (reply: 5)
  376. Protein bands on the bottom of the gel - (reply: 5)
  377. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  378. spot removal from 2D gel and proteomics - help! (reply: 1)
  379. Tube gel IEF problems - Polymerization... (reply: 2)
  380. Pipette tips for gel electrophoresis? - (reply: 4)
  381. Bands in a miRNA gel - (reply: 1)
  382. problems with qiagen gel extraction kit - (reply: 7)
  383. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  384. Gel Electrophoresis band problems - (reply: 3)
  385. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
  386. RNA gel to test DIG labeled RNA probe - (reply: 1)
  387. wacky RNA gel - (reply: 1)
  388. Inseparable insert in gel purification of the vector - (reply: 3)
  389. Problem with protein samples - No bands detected at all on gel (reply: 2)
  390. NuPAGE bis-tris gel transfer buffer question - (reply: 1)
  391. question about bigdye sequencing reaction & capillary electrophoresis - (reply: 3)
  392. using cell lysate to run native gel? - (reply: 1)
  393. Why do gel electrophoresis buffers need an electrolyte? - (reply: 4)
  394. Gel loading dye : sample ratio?? - have to follow strictly? (reply: 6)
  395. Gel electrophoresis problem - (reply: 7)
  396. dna smear in electrophoresis - (reply: 6)
  397. LB and SB electrophoresis media - (reply: 1)
  398. Electrophoresis gel boxes and geldoc system - (reply: 2)
  399. 3T3 cells - Collagen Gel (reply: 1)
  400. Storage of gel extracted DNA before ligation - (reply: 3)
  401. DENATURING AGAROSE GEL FOR RNA - (reply: 1)
  402. Not sure of what % to use for gels - (reply: 6)
  403. Protein identification in 2D gel - (reply: 1)
  404. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  405. Making a 3% agarose gel. - (reply: 4)
  406. no separation of MW marker on gel - (reply: 1)
  407. Expired pre-cast gels - (reply: 4)
  408. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  409. Viewing Gels - (reply: 3)
  410. how to extract protein from agarose? - (reply: 1)
  411. I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
  412. SDS-PAGE gel staining problem - (reply: 1)
  413. Gel loading dye recipe - trying to DIY Qiagen gel loading dye (reply: 4)
  414. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  415. Interpreting a gel - (reply: 3)
  416. Interpreting a Plasmid Profile gel photo - (reply: 3)
  417. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  418. Protein size and gel percentage - (reply: 7)
  419. My acrylimide gel rips. - I need help keeping it frmo ripping all the time (reply: 3)
  420. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  421. RNA gel electrohoresis - (reply: 1)
  422. DNA supercoiling on agarose gel -> reference needed - (reply: 1)
  423. DNA Ladder Issues - DNA Ladder Agarose Gel (reply: 5)
  424. SYBR melting curve vs RT-PCR gel - (reply: 1)
  425. why gel is not running in straight line?? - (reply: 13)
  426. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  427. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
  428. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  429. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  430. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  431. casting agarose gel troubleshooting - smeared bands (reply: 8)
  432. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  433. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  434. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  435. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  436. RNA not visible on gel :( - (reply: 3)
  437. how better is 7Cm 2D gel electrophoresis for separation - is anyone doing that? (reply: 1)
  438. Using DNA in gel extract kept at -20ºC - (reply: 2)
  439. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  440. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
  441. UV-transparent gel tray - Gel electrophoresis (reply: 3)
  442. Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
  443. Fast protocol for RNA agarose gel electrophoresis - (reply: 1)
  444. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
  445. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
  446. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  447. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  448. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  449. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
  450. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
  451. Blue Juice loading dye & Gel extraction kit - HELP :( (reply: 6)
  452. denature gel shrink, help - (reply: 5)
  453. Agarose gel with EtBr exposed to light over night. - (reply: 3)
  454. help-how to extract fluo-labeled DNA from agarose gel - (reply: 1)
  455. strange agarose gel - (reply: 9)
  456. cloudy gel - (reply: 2)
  457. How much RNA is needed to see rRNA on a gel? - (reply: 3)
  458. Gel shift (emsa) with 35S label? - (reply: 5)
  459. A260/230 readings post gel extraction - (reply: 19)
  460. pancreas RNA gel run consult - (reply: 1)
  461. Agarose: DNA/dye mixture doesn't stay in the gel? - Thanks for any and all help received! =] (reply: 12)
  462. cracking gel problem - (reply: 1)
  463. bubbles between gel and glass plate - (reply: 9)
  464. How to extract protein from SDS-PAGE gel? - (reply: 7)
  465. i touch agrose gel which contain ethidium bromide - (reply: 6)
  466. Most strange sds page gel ever!!!!! - (reply: 5)
  467. Gel Purification problem..Help~! - (reply: 7)
  468. migration rates of loading dye in sodium borate gel - (reply: 1)
  469. how to solve fungus problem on SDS PAGE gel - (reply: 4)
  470. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  471. dna not running for electrophoresis - (reply: 9)
  472. sonication in ChIP / nonReverse - nonReverse gel run check ok or not (reply: 1)
  473. bad bad Agarose gel - (reply: 9)
  474. uneven on gel electroforesis - uneven on gel electroforesis (reply: 4)
  475. protein degardation during gel filteration - (reply: 2)
  476. abnormal band in agarose gel - Urgent help required (reply: 3)
  477. Running Polyacrylamide gel in acidic conditions - Adicid denaturing PAGE (with or without SD?) (reply: 1)
  478. proteins stuck at interface between stack and separating gel - (reply: 2)
  479. smear on gel after tca precipitation - (reply: 3)
  480. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  481. Gel extraction help.... desperate! - (reply: 6)
  482. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
  483. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
  484. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  485. separation of dna fragments in agarose gel electrophoresis - (reply: 7)
  486. SDS gel - (reply: 2)
  487. problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
  488. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
  489. prearing gel in advance - (reply: 6)
  490. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  491. PCR and gel purification problem - did I screw this up (reply: 4)
  492. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  493. no band in gel - gel electrophoresis (reply: 1)
  494. Agarose gel electrophoresis troubleshooting - (reply: 37)
  495. Gel Analysis Software - which to use (reply: 3)
  496. elution of dna from gel - (reply: 5)
  497. Agarose gel problem.. plz help - (reply: 11)
  498. Question about DNA electrophoresis - (reply: 1)
  499. Agarose gel electrophoresis problems - (reply: 7)
  500. PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
  501. no 18S band on RNA agarose gel - (reply: 1)
  502. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  503. Freedom Rocker - Save yourself from babysitting your gels! - Save yourself from babysitting your gels! (reply: 2)
  504. gel extraction protocol for small amount of starting material - (reply: 3)
  505. types of gel stains - (reply: 7)
  506. 5' RACE products appearance on 1.5% agarose gel - (reply: 8)
  507. Gel mix, acrylamide/bis, Ammonium persulfate, etc disposal - (reply: 1)
  508. Running gel after RT-PCR - (reply: 5)
  509. Heating RNA samples for electrophoresis - (reply: 3)
  510. Importance of Reducing Agent - Apparent protein size on gel (reply: 2)
  511. small PCR products on agarose gel? - (reply: 8)
  512. Gel Electrophoresis Interpretation - (reply: 3)
  513. protein size gradient gel - (reply: 10)
  514. Tris Glycine gel running settings - (reply: 4)
  515. Problem with "smiling effect" on SDS-PAGE gel - (reply: 11)
  516. Cast Bis Tris Gel with fibers in the wells - (reply: 7)
  517. a doubt about PCR gel purification - (reply: 4)
  518. Best lysis buffer for IEF/ 2D gel? - (reply: 2)
  519. funny patterns on a gel - (reply: 4)
  520. low melting point agarose - (reply: 9)
  521. denaturing proteins in gel - (reply: 1)
  522. Problem with DNA purification from agarose gel - (reply: 3)
  523. Agarose gel cutting - (reply: 8)
  524. Any good software for 2D gel analysis - (reply: 1)
  525. No band in the gel after ligation - (reply: 6)
  526. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  527. miRNA Northern Blot gel - (reply: 4)
  528. Rcovery of proteins from gels - (reply: 2)
  529. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  530. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  531. Proteins not running till the end of the gel - (reply: 2)
  532. How does the protein fixation on SDS gel work? - (reply: 3)
  533. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
  534. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  535. Gel purification of vector DNA - low yield - (reply: 26)
  536. gel purification or ligation problem - (reply: 3)
  537. gel shift in western blot - (reply: 3)
  538. Problem with running SDS page gel - (reply: 6)
  539. RNA electrophoresis - (reply: 3)
  540. electrophoresis problem - (reply: 1)
  541. Which Electrophoresis Gel documentation system you like - (reply: 5)
  542. agarose gels - (reply: 1)
  543. Different dyes for gel electrophoresis - (reply: 9)
  544. Is immunoblotting possible after the gel has been silver stained - (reply: 2)
  545. protein disapeared from gel! - (reply: 11)
  546. Loading samples into gel: How much was it? - (reply: 9)
  547. strange double band in electrophoretic pattern - (reply: 2)
  548. 2d gel analysis software??? - (reply: 6)
  549. Gel Electrophoresis.... - ....some samples running the wrong direction (reply: 5)