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Electrophoresis Related Discussions
  1. Gel for my ligation - (reply: 1)
  2. first time running DNA polyacrylamide TBE gel - (reply: 4)
  3. DNA gel elctrophoresis problem - (reply: 3)
  4. Relative humidity in the electrophoresis lab - (reply: 3)
  5. Ladder and proteins do not migrate into stacking gel - (reply: 5)
  6. Transforming dna from agarose gel - (reply: 3)
  7. page electrophoresis - (reply: 2)
  8. Low A260/230 after gel extraction - (reply: 1)
  9. Disparity between nanodrop and gel - (reply: 2)
  10. RNA agarose gel electrophoresis came up empty - (reply: 10)
  11. Sequencing gels equipment query - (reply: 2)
  12. Measuring pcr fragments in a gel - (reply: 1)
  13. gel electrophoresis - (reply: 4)
  14. Top of agarose gel blank? - (reply: 3)
  15. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
  16. EtBr decontamination of gel doc system - (reply: 2)
  17. Electrophoresis Imaging - (reply: 1)
  18. Electrophoresis after PCR : too many bands - (reply: 6)
  19. DNA virus purification from agarose failed! - (reply: 7)
  20. Ligation failure? I ran some on a gel - (reply: 6)
  21. Why does my gel have streaks instead of bands? - (reply: 2)
  22. Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
  23. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
  24. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
  25. Electrophoresis samples and volumes - (reply: 1)
  26. Native PAGE problem - not entering gel enough - (reply: 5)
  27. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  28. Problems with wells on SDS PAGE gels - (reply: 3)
  29. Checking RNA integrity by Denaturing Gel - (reply: 2)
  30. Gel Electrophoresis: Help needed - (reply: 15)
  31. Altering gel voltage and run time. - (reply: 9)
  32. BrdU incorporated DNA- move slower during electrophoresis? - (reply: 2)
  33. 3D printing of (horizontal, agarose) gel trays, and other lab equipment - (reply: 6)
  34. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  35. polyacrylamide gel with sybr green staining - (reply: 1)
  36. Weirdest thing with DNA dye and agarose gel - (reply: 4)
  37. PAGE loading dye vs agarose loading dye - (reply: 1)
  38. Is NuPAGE Bis-Tris gel for native or denaturing protein? - (reply: 1)
  39. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  40. Smeary bands on agarose gel (also the ladder) - (reply: 2)
  41. manual 2D electrophoresis - (reply: 8)
  42. Is there any alternative to Formamide in RNA Gel Electrophoresis? - (reply: 1)
  43. Sudden problems with Separating Proteins using SDS Gel - (reply: 4)
  44. Multiple Bands after gel extraction - (reply: 4)
  45. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  46. UV analysis of gel - (reply: 2)
  47. DNA extraction from the gel - low yield - (reply: 2)
  48. Plasmid digestion and gel purification using HindIII - (reply: 3)
  49. agarose sterilization - (reply: 3)
  50. Agarose bead size in affinity chromatography - (reply: 2)
  51. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  52. Perculiar contamination of agarose gel combs? - (reply: 3)
  53. MS-compatible methods for blocking agarose beads - (reply: 1)
  54. 2D gel analysis software - (reply: 1)
  55. Gel purification (DNA) - (reply: 1)
  56. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  57. Running undigested plasmids on gel - (reply: 2)
  58. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  59. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
  60. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ri - (reply: 1)
  61. Gradient Gel Electrophoresis - (reply: 3)
  62. SDS-PAGE resolving gel not completely set - (reply: 4)
  63. Problem with SDS-PAGE gel - (reply: 13)
  64. gel shrinkage ??? - (reply: 2)
  65. Wavy Lines in Gel - (reply: 2)
  66. Protein extraction for 2D electrophoresis - (reply: 2)
  67. Casein gel zymography - (reply: 1)
  68. interpreting RNA agarose gel results? - (reply: 1)
  69. smear in gel electrophoresis after PCR - (reply: 2)
  70. No DNA after gel extraction - (reply: 9)
  71. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  72. LMW proteins not transfer of gel to membrane - (reply: 1)
  73. purify protein from native gel - (reply: 1)
  74. Weird gel banding for apoptosis - (reply: 3)
  75. Undefined bands in Gel - (reply: 9)
  76. Electrophoresis - (reply: 1)
  77. Stain in electrophoresis is going from + to - - (reply: 3)
  78. agarose gel electrophoresis of RNA - (reply: 1)
  79. EtBr in agarose gel and running buffer - (reply: 3)
  80. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
  81. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
  82. Agarose gel bands gone (for gel extraction) - (reply: 2)
  83. About Gel supershift - (reply: 1)
  84. siRNA Agarose gel electrophoresis - Protocol - (reply: 1)
  85. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  86. cDNA test on 1% agarose gel - (reply: 3)
  87. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  88. Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
  89. Help with bad electrophoresis run. - (reply: 3)
  90. Quantify DNA in gel electrophoresis bands - (reply: 7)
  91. How to make gradient gel (10-20%), how to control the speed of flow? - (reply: 4)
  92. Low yield PCR product after gel purification - (reply: 8)
  93. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
  94. Straight bands on DNA gel - (reply: 3)
  95. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  96. Electrophoresis combs - Mini-plus vs Midi - (reply: 8)
  97. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  98. reason of vertical streaks on gels - (reply: 1)
  99. Reused glass bottles to make ethidium bromide gels - (reply: 1)
  100. Just built a gel chamber, blue LED transilluminator, ect.. selling - (reply: 2)
  101. PCR product running on agarose gel - (reply: 32)
  102. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  103. Blue fluorescent color of agarose gel in UV - (reply: 7)
  104. gel shift assay by chemilumniscence. - (reply: 1)
  105. Electrophoresis problem , please help pals ! - (reply: 8)
  106. Western blot question- High % Gel/Low MW Protein - (reply: 7)
  107. too much DNA on agarose gel - (reply: 9)
  108. polyacrylamide RNA gel - (reply: 4)
  109. isoelctric gels hard to stain? - (reply: 2)
  110. protein sample floating out of the tricine gel - (reply: 3)
  111. Protein Purification from Polyacrylamide Gels - (reply: 3)
  112. bands position in agarose gel - (reply: 4)
  113. Protein degradation on 2D gels - (reply: 2)
  114. SDS PAGE gel storing - (reply: 3)
  115. White bands on Cy3 gels - (reply: 1)
  116. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
  117. mini printer for printing gel images to insert into lab book - (reply: 13)
  118. SDS PAGE gels - (reply: 5)
  119. Premixing gel solutions - (reply: 2)
  120. Odd Bands on Gel - (reply: 3)
  121. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
  122. qPCR: band in gel, but super high Cq? - (reply: 10)
  123. How to avoid gel breakage after electrophoresis - (reply: 5)
  124. A good UV transilluminator (electrophoresis) - (reply: 4)
  125. Agarose gell electrophoresis - (reply: 1)
  126. Please look at my gel picture, poor EthBr staining - (reply: 2)
  127. Good loading control for native gels? - (reply: 1)
  128. Pattern/cloud appearing on agarose gel - (reply: 15)
  129. no product on gel after restriction digestion - (reply: 3)
  130. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
  131. TAU gel electrophoresis of histones - (reply: 4)
  132. Problems with Electrophoresis of RNA - (reply: 3)
  133. Problems with having to run the gels for so long - (reply: 14)
  134. Ethidium Bromide Agarose Gel Hazard - (reply: 6)
  135. Single Plasmid Gel Band - (reply: 1)
  136. Buffer in Gel - (reply: 6)
  137. how do GST-tags affect gel filtration results? - (reply: 5)
  138. problems with recovery of dna from gel and ligation - (reply: 5)
  139. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
  140. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  141. QIAquick Gel Extraction - (reply: 2)
  142. agarose gel electrophoresis - (reply: 5)
  143. Uneven bands denaturing agarose - (reply: 2)
  144. Acid urea gel electrophoresis (AUGE) - (reply: 7)
  145. storing the loaded gel before running it - (reply: 7)
  146. What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely - (reply: 7)
  147. Smear on agarose gel from QIAGEN extracted plant samples - (reply: 1)
  148. E gels and the E gel opener - (reply: 1)
  149. SYBR gold post-stain makes blurry gels - (reply: 1)
  150. Dyes for RNA in Urea-polyacrylamide gel - (reply: 1)
  151. Coomassie stain forzen and thawed gel? - (reply: 1)
  152. PCR band moves lower than its size on agarose gel - (reply: 3)
  153. IEF gel detaching during run - (reply: 7)
  154. Agarose gel electrophoresis of RNA - (reply: 5)
  155. What causes surface fluorescence on agarose gels? - (reply: 6)
  156. Problems with PAGE gels - (reply: 1)
  157. oligo continually appears less on gel compared to other same load - (reply: 2)
  158. can coommassie stained gel be western blot - (reply: 5)
  159. carpet in gels for PCR products - (reply: 2)
  160. What % native urea gel do I use for Fab and F(ab')2? - (reply: 2)
  161. protein extraction from GEL - (reply: 5)
  162. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  163. Odd gel run following PCR - (reply: 28)
  164. Problem with visibility on third comb in gels - (reply: 4)
  165. how much time for prepared gel to keep bfore loading? - (reply: 5)
  166. Transfer of proteins from gel to PVDF for short period - (reply: 3)
  167. Product help: Inexpensive Agarose Gel - (reply: 1)
  168. what is your DNA concentration after gel extraction? - (reply: 5)
  169. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  170. Vertical Vs horizontal electrophoresis - (reply: 3)
  171. RNase h electrophoresis - (reply: 1)
  172. RNA degradation problem, only one band on the gel - (reply: 3)
  173. rtPCR working but the elongated product wont gel! - (reply: 1)
  174. Using 4-year old precast gels - (reply: 4)
  175. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  176. Using out-dated equipment to image agarose gel samples-help - (reply: 1)
  177. Second round of PCR after gel extraction fails miserably - (reply: 3)
  178. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
  179. No bands appearing in DNA gel electrophoresis - (reply: 1)
  180. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
  181. What percentage should I use for a stacking gel? - (reply: 1)
  182. What percentage gel should I make? - (reply: 1)
  183. White big cloud in agar gel electrophoresis - (reply: 13)
  184. Signal stuck on top of seperating gel - (reply: 1)
  185. SDS-PAGE gel and running buffer - (reply: 3)
  186. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  187. Agarose Gel - (reply: 1)
  188. Denaturing agent Acrylamide-bisacruylamide gel - (reply: 6)
  189. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
  190. Vertical agarose gel electrophoresis for DNA - (reply: 1)
  191. Running Primers on Agarose Gel - (reply: 6)
  192. strange bands shape agarose gel - (reply: 2)
  193. Estimating size of PCR products in a agarose gel - (reply: 4)
  194. Ladders not resolving on agarose gel - (reply: 6)
  195. IEF migration to middle of gel, no separation - (reply: 2)
  196. how to dissolve Polyacrylamide gel? - (reply: 8)
  197. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
  198. Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
  199. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
  200. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  201. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
  202. restaining of coommassie destained gel - (reply: 1)
  203. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  204. how to make nondenaturing gradient gel - (reply: 2)
  205. Pulsed Field Gel Electrophoresis - (reply: 4)
  206. Sphere staining protocol (agarose) - (reply: 3)
  207. How to interpret this agarose gel? - (reply: 6)
  208. Ligation gel picture interpretation - (reply: 4)
  209. Is there an optimum % agarose or... "more is better"? - (reply: 2)
  210. Western gels running super slow! - (reply: 3)
  211. How to use the OWL Gel Casting System? - (reply: 2)
  212. RNA electrophoresis - (reply: 4)
  213. DNA ladder running faster than samples during agarose gel electrophoresis - (reply: 1)
  214. Troubles with casting Gradient Gels - (reply: 2)
  215. Polyclonal antibody production using SDS-PAGE gel fragment - (reply: 3)
  216. total RNA Extraction gel control - (reply: 1)
  217. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
  218. PAGE gel recipes - (reply: 1)
  219. SB buffer and agarose: Fragments less than 100 bp - (reply: 1)
  220. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  221. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
  222. PEG 8000 precipitation/ gel electrophoresis - (reply: 5)
  223. Software to construct a cladogram from a gel - (reply: 2)
  224. Empty agarose gel after electrophoresis - (reply: 1)
  225. Bright bands of PCR products stick in gel wells - (reply: 1)
  226. DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
  227. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  228. polyacrylamide gel problem....slipping - (reply: 5)
  229. Anoyone knows a good reliable gel documentation system? - (reply: 4)
  230. how to seperate double-digestion products on agarose gel - (reply: 14)
  231. Samples leaking out of wells in gel - (reply: 2)
  232. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  233. syto9 for DNA gel electrophoresis - (reply: 1)
  234. I can't see anything in my gel. I need help - (reply: 1)
  235. Help with DNA acrylamide gel - leaking and errors during gel running - (reply: 4)
  236. Bradford reagent for staining gels? - (reply: 1)
  237. wrong size in agarose gel, right size in sequencing - (reply: 7)
  238. 1D analysis electrophoresis software for automated labelling - (reply: 3)
  239. Weird DNA electrophoresis result - (reply: 4)
  240. DNA migrate differently in agarose vs PAGE gel - (reply: 4)
  241. RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
  242. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  243. poor seal between stacking a seperating gels - (reply: 4)
  244. discarding acrylamide gels?? - (reply: 2)
  245. Agarose - (reply: 7)
  246. IEF technique utilizing Multiphor II Electrophoresis System - I am totally a newbie (reply: 9)
  247. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
  248. Problem with protein resolving in electrophoresis - (reply: 7)
  249. RNA degradation during electrophoresis?? - (reply: 2)
  250. gel question - (reply: 3)
  251. electrophoresis rflp help - (reply: 1)
  252. Stacking gel for DNA PAGE - (reply: 2)
  253. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  254. Bioanalyzer and RNA gel - (reply: 2)
  255. EMSA + WB - Transferring 6% native gel (reply: 1)
  256. SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
  257. storing SDS-PAGE gels for W.Blot - (reply: 7)
  258. acrylamide gel for low molecular weight protein - (reply: 2)
  259. how many samples should i prepare? - about 2 dimensional electrophoresis (reply: 2)
  260. The Best Gel Extraction Kits - The Best Gel Extraction Kits (reply: 9)
  261. agarose gel has melten - (reply: 4)
  262. Dna after gel extraction, single or double stranded? - (reply: 1)
  263. Checking RNA on gel after DIG labeling - (reply: 1)
  264. Gels 'blobby' - (reply: 2)
  265. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
  266. Gel filtration chromatography - for fractionation of lysate (reply: 4)
  267. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  268. Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
  269. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
  270. SDS-PAGE gel wont separate small proteins - (reply: 5)
  271. SDS Acrylamide Gel Equipement - Need to purchase best and cheapest new equipment (reply: 3)
  272. PCR Gel nonspecific band - (reply: 13)
  273. Single-digested vector shifts downward on gel - (reply: 1)
  274. Funny DNA bands on Agarose gels - (reply: 6)
  275. leaving gel in buffer ! - (reply: 3)
  276. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
  277. 2d gel technique - (reply: 2)
  278. Gel Extraction using buffer QG then phenol/chl - Can I? (reply: 1)
  279. Tricine-SDS gels blotting problems - (reply: 4)
  280. native PAGE gel - (reply: 2)
  281. Method to dissolve agarose gel? - (reply: 2)
  282. Problem loading DNA / agarose gel - (reply: 11)
  283. Which buffer do you use for gel electrophoresis? - (reply: 9)
  284. Agarose Gel Disposal - (reply: 13)
  285. Problem Viewing bands in Agarose Gel - (reply: 6)
  286. Agarose gel loading sample pops out - Sample pops out when loading gel (reply: 3)
  287. agarose gel electrophoresis - (reply: 3)
  288. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  289. No bands on agarose gel after restriction digestion. - (reply: 10)
  290. Gel filtration Chromatography - (reply: 1)
  291. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
  292. Stacking Gel Polymerization - (reply: 4)
  293. not "that" 2D gel - (reply: 2)
  294. Extra band on gel after miniprep - (reply: 6)
  295. 3 distinct bands of gDNA on gel - (reply: 2)
  296. Dry protein gels without cracking - (reply: 5)
  297. power supply electrophoresis - (reply: 11)
  298. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  299. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
  300. resolving gel pourcentage - (reply: 3)
  301. Total RNA only produce 1 band in gel - (reply: 2)
  302. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  303. How to modify electrophoresis chamber - (reply: 3)
  304. NuPAGE Gel get smaller after staining o/n - (reply: 1)
  305. RNA in native gel - (reply: 3)
  306. gel documentation system broken - any ideas how to fix? (reply: 2)
  307. How to reduce electrophoresis times - Impatient me needs faster results (reply: 16)
  308. question about plating cells - gel or no gel? (reply: 2)
  309. DNA, Gel - Possible reasons for DNA Gel Smear (reply: 12)
  310. TBE/Agarose Gel Concentrations - (reply: 3)
  311. Agarose gel to separate btween 4800bp and 4850bp - (reply: 3)
  312. Electrophoretic Mobility Shift Assay - no free probes shown in samples (reply: 3)
  313. Denaturing gel electrophoresis for qPCR - (reply: 3)
  314. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  315. Lithium electrophoresis buffer - Why does it not work? (reply: 2)
  316. Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
  317. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
  318. PCR, using gel extracted band as a template - (reply: 4)
  319. Which gel concentration - (reply: 4)
  320. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  321. Combining forward and reverse primer gives different size on gel - (reply: 2)
  322. Agarose Gel Electrophoreses - What makes it fluoresce? - (reply: 3)
  323. TBE Acrylamide/Formamide gels running slowly - (reply: 4)
  324. microinjection of zebrafish - the agarose plate - where to buy the mould for the grooves (reply: 1)
  325. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
  326. making gel electrophoresis - (reply: 1)
  327. Running a western with cell media in gels? - (reply: 11)
  328. Gel electrophoresis - (reply: 4)
  329. running problems SDS-PAGE gel (zymography) - (reply: 4)
  330. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  331. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  332. Estimation of DNA Quantity on an Agarose Gel - (reply: 5)
  333. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  334. Electrophoresis: sometimes mA=0 - (reply: 3)
  335. low melt agarose - (reply: 1)
  336. Agarose Gel electrophoresis - (reply: 2)
  337. Problems with qiaquick gel purification kit - cloning (reply: 10)
  338. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
  339. strange problem in PAGE gel - (reply: 12)
  340. Anomalous asymmetrical gel - (reply: 1)
  341. Noisy fluorescent gels - (reply: 1)
  342. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  343. got smear on the PCR gel !!! - (reply: 4)
  344. MaestroGen UltraSlim LED illuminator - for DNA gel (reply: 1)
  345. Gel Electrophoresis Question - (reply: 12)
  346. Backwards electrophoresis electrodes - Can you dig it? (reply: 1)
  347. resolving and stucking gel solutions - (reply: 2)
  348. SDS PAGE - Bubble inside the Gel (reply: 5)
  349. serum separation: transparent gel prevents serum collection! - (reply: 2)
  350. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  351. checking sonication using chelex and agarose gel - (reply: 10)
  352. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
  353. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  354. uncut vector contamination in gel purified band - (reply: 2)
  355. Agarose gel electrophresis - (reply: 3)
  356. Genomic DNA electrophoresis? - (reply: 1)
  357. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
  358. polyacrylamide gel storage - (reply: 3)
  359. RNA degradation in collagen gels - (reply: 1)
  360. making gel in advance - to save time (reply: 7)
  361. Agarose gel bands smear/ drag problem - (reply: 4)
  362. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  363. gel does not polymerize - (reply: 12)
  364. Gel Storage for PAGE - Advance casting of polyacrylamide gels (reply: 2)
  365. How many volts/milliamps to run gel at? - (reply: 2)
  366. EMSA gels - (reply: 2)
  367. 6.7%Polyacrylamide/50% Urea gel - (reply: 1)
  368. Migration Distance on a Gel - (reply: 2)
  369. Varied resolution in the same gel - (reply: 2)
  370. Large DNA extraction from an agarose gel - (reply: 8)
  371. Strange SDS gel after Western Blotting - (reply: 1)
  372. Lost protein after gel filtration - Gel Filtration (reply: 6)
  373. Electrophoresis problems - (reply: 6)
  374. how to do gel documentation - (reply: 2)
  375. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
  376. Does agarose go bad? - (reply: 14)
  377. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
  378. Problem- wierd 2D gel image - (reply: 5)
  379. Agarose gel electrophoresis question - (reply: 1)
  380. H3 in tris tricine gels - (reply: 2)
  381. Microcentrifuge Gel Filtration Question - (reply: 4)
  382. SB Buffer and Page Electrophoresis - (reply: 3)
  383. What causes this offset band on RIPA gel? - (reply: 4)
  384. Running real time pcr product on gel?.. - (reply: 4)
  385. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
  386. DNA quantification after gel purification - (reply: 6)
  387. Please help me to choose right Gel imager!!! - (reply: 2)
  388. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  389. Breast Feeding and Ethiduim Bromide - Can you make agarose gels? (reply: 10)
  390. Western blot problems - No or vey little transfer, expanding gel in transfer (reply: 5)
  391. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  392. Problem with gel extraction of DNA. - (reply: 1)
  393. GHOST in Electrophoresis - (reply: 18)
  394. Akrylamide Gels - (reply: 2)
  395. gel filtration.. - (reply: 1)
  396. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
  397. Making gels in advance - (reply: 2)
  398. No protein spots in 2D gel - Silver staining! - (reply: 17)
  399. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
  400. Probelm with gel filtration - (reply: 4)
  401. 3% NuSieve gel electrophoresis - Why 1kb ladder doesn't move well? (reply: 3)
  402. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  403. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  404. Agarose / SDS Page - (reply: 2)
  405. GEL SMEAR - (reply: 4)
  406. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
  407. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  408. problem about DNA electrophoresis - the DNAs become two layers in the gel (reply: 4)
  409. No SDS in gels - (reply: 12)
  410. densidometric analysis with scans - no gels available!!! (reply: 4)
  411. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  412. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  413. Extraction of DNA smaller than 100 bp from Agarose Gel - (reply: 6)
  414. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  415. How to view 75 bp DNA in gel - (reply: 4)
  416. gel documentation sysytem - (reply: 7)
  417. Gel Running Crooked?? Please help.. - (reply: 7)
  418. peptide extraction tricine gel - (reply: 1)
  419. the ChIP agarose gel results - Should the input always be more than the IP one? (reply: 2)
  420. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
  421. DNA damage in gel - (reply: 8)
  422. Software for labeling gel picture - (reply: 1)
  423. Can i use agarose gel to seperate 80bp - (reply: 2)
  424. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  425. Re-using unloaded agarose gel - (reply: 6)
  426. PCR product appear two close band in my gel - (reply: 15)
  427. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  428. Destaining a coomassie sttained gel - (reply: 14)
  429. 5% agarose - (reply: 4)
  430. Too much failure in electrophoresis, am I doing the gel correctly? - (reply: 10)
  431. Desperate! Loss of basic proteins on 2D gels - (reply: 5)
  432. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  433. Sodium borate buffer gel problems - (reply: 5)
  434. help with gel shift assay - (reply: 1)
  435. Run PCR amplifications in agarose gels - (reply: 3)
  436. Aggregated samples at the top of SDS-PAGE gel - (reply: 3)
  437. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  438. Silver stained 2-d gel BLOB!!! - (reply: 1)
  439. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
  440. Problems with small peptide electrophoresis - (reply: 2)
  441. EMSA gel problem - (reply: 2)
  442. Harvesting cells off of collagen gels - (reply: 1)
  443. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  444. Simple gel question - (reply: 7)
  445. DNA agarose gel electrophoresis - sum1 can teach me interpret this gel image.. (reply: 3)
  446. 2 d gel electrophoresis consumable - any brand? (reply: 1)
  447. Pulse field gel electrophoresis - (reply: 2)
  448. PCR bands on gel electrophoresis - (reply: 5)
  449. to keep sds-pa gels - (reply: 2)
  450. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
  451. Hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - Well,actually I want to make some homemade agarose beads,which haven't (reply: 5)
  452. Smearing on gel in PCR products - (reply: 5)
  453. Protein bands on the bottom of the gel - (reply: 5)
  454. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  455. spot removal from 2D gel and proteomics - help! (reply: 1)
  456. Tube gel IEF problems - Polymerization... (reply: 2)
  457. Pipette tips for gel electrophoresis? - (reply: 4)
  458. Bands in a miRNA gel - (reply: 1)
  459. problems with qiagen gel extraction kit - (reply: 7)
  460. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  461. Gel Electrophoresis band problems - (reply: 3)
  462. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
  463. RNA gel to test DIG labeled RNA probe - (reply: 1)
  464. wacky RNA gel - (reply: 1)
  465. Inseparable insert in gel purification of the vector - (reply: 3)
  466. Problem with protein samples - No bands detected at all on gel (reply: 2)
  467. NuPAGE bis-tris gel transfer buffer question - (reply: 1)
  468. question about bigdye sequencing reaction & capillary electrophoresis - (reply: 3)
  469. using cell lysate to run native gel? - (reply: 1)
  470. Why do gel electrophoresis buffers need an electrolyte? - (reply: 4)
  471. Gel loading dye : sample ratio?? - have to follow strictly? (reply: 6)
  472. Gel electrophoresis problem - (reply: 7)
  473. dna smear in electrophoresis - (reply: 6)
  474. LB and SB electrophoresis media - (reply: 1)
  475. Electrophoresis gel boxes and geldoc system - (reply: 2)
  476. 3T3 cells - Collagen Gel (reply: 1)
  477. Storage of gel extracted DNA before ligation - (reply: 3)
  478. DENATURING AGAROSE GEL FOR RNA - (reply: 1)
  479. Not sure of what % to use for gels - (reply: 6)
  480. Protein identification in 2D gel - (reply: 1)
  481. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  482. Making a 3% agarose gel. - (reply: 4)
  483. no separation of MW marker on gel - (reply: 1)
  484. Expired pre-cast gels - (reply: 4)
  485. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  486. Viewing Gels - (reply: 3)
  487. how to extract protein from agarose? - (reply: 1)
  488. I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
  489. SDS-PAGE gel staining problem - (reply: 1)
  490. Gel loading dye recipe - trying to DIY Qiagen gel loading dye (reply: 4)
  491. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  492. Interpreting a gel - (reply: 3)
  493. Interpreting a Plasmid Profile gel photo - (reply: 3)
  494. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  495. Protein size and gel percentage - (reply: 7)
  496. My acrylimide gel rips. - I need help keeping it frmo ripping all the time (reply: 3)
  497. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  498. RNA gel electrohoresis - (reply: 1)
  499. DNA supercoiling on agarose gel -> reference needed - (reply: 1)
  500. DNA Ladder Issues - DNA Ladder Agarose Gel (reply: 5)
  501. SYBR melting curve vs RT-PCR gel - (reply: 1)
  502. why gel is not running in straight line?? - (reply: 13)
  503. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  504. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
  505. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  506. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  507. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  508. casting agarose gel troubleshooting - smeared bands (reply: 8)
  509. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  510. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  511. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  512. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  513. RNA not visible on gel :( - (reply: 3)
  514. how better is 7Cm 2D gel electrophoresis for separation - is anyone doing that? (reply: 1)
  515. Using DNA in gel extract kept at -20C - (reply: 2)
  516. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  517. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
  518. UV-transparent gel tray - Gel electrophoresis (reply: 3)
  519. Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
  520. Fast protocol for RNA agarose gel electrophoresis - (reply: 1)
  521. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
  522. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
  523. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  524. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  525. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  526. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
  527. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
  528. Blue Juice loading dye & Gel extraction kit - HELP :( (reply: 6)
  529. denature gel shrink, help - (reply: 5)
  530. Agarose gel with EtBr exposed to light over night. - (reply: 3)
  531. help-how to extract fluo-labeled DNA from agarose gel - (reply: 1)
  532. strange agarose gel - (reply: 9)
  533. cloudy gel - (reply: 2)
  534. How much RNA is needed to see rRNA on a gel? - (reply: 3)
  535. Gel shift (emsa) with 35S label? - (reply: 5)
  536. A260/230 readings post gel extraction - (reply: 19)
  537. pancreas RNA gel run consult - (reply: 1)
  538. Agarose: DNA/dye mixture doesn't stay in the gel? - Thanks for any and all help received! =] (reply: 12)
  539. cracking gel problem - (reply: 1)
  540. bubbles between gel and glass plate - (reply: 9)
  541. How to extract protein from SDS-PAGE gel? - (reply: 7)
  542. i touch agrose gel which contain ethidium bromide - (reply: 6)
  543. Most strange sds page gel ever!!!!! - (reply: 5)
  544. Gel Purification problem..Help~! - (reply: 7)
  545. migration rates of loading dye in sodium borate gel - (reply: 1)
  546. how to solve fungus problem on SDS PAGE gel - (reply: 4)
  547. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  548. dna not running for electrophoresis - (reply: 9)
  549. sonication in ChIP / nonReverse - nonReverse gel run check ok or not (reply: 1)
  550. bad bad Agarose gel - (reply: 9)
  551. uneven on gel electroforesis - uneven on gel electroforesis (reply: 4)
  552. protein degardation during gel filteration - (reply: 2)
  553. abnormal band in agarose gel - Urgent help required (reply: 3)
  554. Running Polyacrylamide gel in acidic conditions - Adicid denaturing PAGE (with or without SD?) (reply: 1)
  555. proteins stuck at interface between stack and separating gel - (reply: 2)
  556. smear on gel after tca precipitation - (reply: 3)
  557. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  558. Gel extraction help.... desperate! - (reply: 6)
  559. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
  560. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
  561. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  562. separation of dna fragments in agarose gel electrophoresis - (reply: 7)
  563. SDS gel - (reply: 2)
  564. problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
  565. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
  566. prearing gel in advance - (reply: 6)
  567. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  568. PCR and gel purification problem - did I screw this up (reply: 4)
  569. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  570. no band in gel - gel electrophoresis (reply: 1)
  571. Agarose gel electrophoresis troubleshooting - (reply: 37)
  572. Gel Analysis Software - which to use (reply: 3)
  573. elution of dna from gel - (reply: 5)
  574. Agarose gel problem.. plz help - (reply: 11)
  575. Question about DNA electrophoresis - (reply: 1)
  576. Agarose gel electrophoresis problems - (reply: 7)
  577. PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
  578. no 18S band on RNA agarose gel - (reply: 1)
  579. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  580. Freedom Rocker - Save yourself from babysitting your gels! - Save yourself from babysitting your gels! (reply: 2)
  581. gel extraction protocol for small amount of starting material - (reply: 3)
  582. types of gel stains - (reply: 7)
  583. 5' RACE products appearance on 1.5% agarose gel - (reply: 8)
  584. Gel mix, acrylamide/bis, Ammonium persulfate, etc disposal - (reply: 1)
  585. Running gel after RT-PCR - (reply: 5)
  586. Heating RNA samples for electrophoresis - (reply: 3)
  587. Importance of Reducing Agent - Apparent protein size on gel (reply: 2)
  588. small PCR products on agarose gel? - (reply: 8)
  589. Gel Electrophoresis Interpretation - (reply: 3)
  590. protein size gradient gel - (reply: 10)
  591. Tris Glycine gel running settings - (reply: 4)
  592. Problem with "smiling effect" on SDS-PAGE gel - (reply: 11)
  593. Cast Bis Tris Gel with fibers in the wells - (reply: 7)
  594. a doubt about PCR gel purification - (reply: 4)
  595. Best lysis buffer for IEF/ 2D gel? - (reply: 2)
  596. funny patterns on a gel - (reply: 4)
  597. low melting point agarose - (reply: 9)
  598. denaturing proteins in gel - (reply: 1)
  599. Problem with DNA purification from agarose gel - (reply: 3)
  600. Agarose gel cutting - (reply: 8)
  601. Any good software for 2D gel analysis - (reply: 1)
  602. No band in the gel after ligation - (reply: 6)
  603. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  604. miRNA Northern Blot gel - (reply: 4)
  605. Rcovery of proteins from gels - (reply: 2)
  606. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  607. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  608. Proteins not running till the end of the gel - (reply: 2)
  609. How does the protein fixation on SDS gel work? - (reply: 3)
  610. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
  611. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  612. Gel purification of vector DNA - low yield - (reply: 26)
  613. gel purification or ligation problem - (reply: 3)
  614. gel shift in western blot - (reply: 3)
  615. Problem with running SDS page gel - (reply: 6)
  616. RNA electrophoresis - (reply: 3)
  617. electrophoresis problem - (reply: 1)
  618. Which Electrophoresis Gel documentation system you like - (reply: 5)
  619. agarose gels - (reply: 1)
  620. Different dyes for gel electrophoresis - (reply: 9)
  621. Is immunoblotting possible after the gel has been silver stained - (reply: 2)
  622. protein disapeared from gel! - (reply: 11)
  623. Loading samples into gel: How much was it? - (reply: 9)
  624. strange double band in electrophoretic pattern - (reply: 2)
  625. 2d gel analysis software??? - (reply: 6)
  626. Gel Electrophoresis.... - ....some samples running the wrong direction (reply: 5)