H3 in tris tricine gels - (Sep/26/2010 )
I would like to know if histone H3 migrates at a different height in tris tricine gels... In other words, is it possible that H3 appears in WB as a 25 KDa band?
Histones are very positively charged. Histone H2B and H3 runs between 20-25 kDa in my gels (real MW is ~ 15kDa). Also, many pre-stained protein markers are not very accurate for MW detection. If you are not sure you are detecting histone, run some purified histone in your gel. Good luck!
Thank you very much! I have already got some purified histone H3, let's see if I'm lucky this time!