Weird gel banding for apoptosis - (Jun/30/2013 )
I'm currently doing an apoptosis assay using the protocol from http://www.celldeath.de/apometh/dnaladd.html.
And when run on gel, the results I got had some smearing above the well. Anyone has encountered this before and know why did this happened?
L1: 1kb ladder
L2: Negative control (no apoptosis)
L3: Positive control (apoptosis present)
Your DNA has stopped in wells because Proteinase K has not done its job completely.
Do you have any idea about the present of smearing above the well too?
I don't think that what you see above the well is smearing, but just the Ethidum bromide running in the opposite direction to the DNA. (Assuming you are using EtBr).