Empty agarose gel after electrophoresis - (Oct/16/2011 )
I was wondering if you could help me to figure out what is wrong. I ran a gel on Friday afternoon for 40 minutes- 100V, stained it using ethidium bromide for 10 minutes and when I check the gel it was EMPTY:( I couldn't even see the ladder. The gel was 1%. What do you think happened??
Ran the gel backwards? Used water to make the gel instead of buffer? Missing loading buffer so you DNA floated out of the wells?