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Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (May/24/2012 )

Dear all,

Why do my bands sometimes appear like the following picture when i run agarose gel electrophoresis?

(somehow i fail to upload the photo in this forum, so please refer to

These are supposedly sharp bands but they just won't separate well. Sometimes I re-run agarose gel and I got sharp bands!


You told us absolutely nothing about this gel.