agarose gel electrophoresis of RNA - (May/14/2013 )
i did agarose gel electrophoresis of RNA(1% agarose gel. instead of proper bands i got smearing of RNA ..I couldnot found the reason why it happens
please give suggestion...
more information needed, but...
are you running total rna and seeing bands and a smear? if so, that is normal. the bands would be rrna and the smear would be mrna.
are you seeing only a smear? are you running mrna (a smear is normal) or total rna? are you running a denaturing gel? if so, are you using formamide (in either the gel or loading buffer or both)? if so, are you sure the formamide hasn't decomposed?