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agarose gel electrophoresis of RNA - (May/14/2013 )

Hello Everyone,

i did agarose gel electrophoresis of RNA(1% agarose gel. instead of proper bands i got smearing of RNA ..I couldnot found the reason why it happens

please give suggestion...

-Prashant Alte-

more information needed, but...

are you running total rna and seeing bands and a smear? if so, that is normal. the bands would be rrna and the smear would be mrna.

are you seeing only a smear? are you running mrna (a smear is normal) or total rna? are you running a denaturing gel? if so, are you using formamide (in either the gel or loading buffer or both)? if so, are you sure the formamide hasn't decomposed?