Protocol Online logo
Top : New Forum Archives (2009-): : Protein and Proteomics

how many samples should i prepare? - about 2 dimensional electrophoresis (Jun/17/2011 )

Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.

May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?

Thanks!

-yimao-

your request misses the aim of your 2D study so it is difficult to answer


generally you should run at least n=3 gels of 3 different cell cultures



yimao on Sat Jun 18 05:20:26 2011 said:


Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.

May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?

Thanks!

-Inmost sun-

oh, sorry about my bad expression.

i am trying to compare the protein expression differences made by culture cell in difference drug treat conditions.

do you mean i need to run 3*3=9 gels for one cell culture condition or 1*3=3 gels?

thx~

Inmost sun on Sun Jun 19 10:55:51 2011 said:


your request misses the aim of your 2D study so it is difficult to answer


generally you should run at least n=3 gels of 3 different cell cultures



yimao on Sat Jun 18 05:20:26 2011 said:


Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.

May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?

Thanks!

-yimao-