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Single Plasmid Gel Band - (Sep/18/2012 )

Can (in any misterious special conditions) a non-digested plasmid appear as a single band (without the coiled states) in a ordinary agarose electrophoresis gel!?
I heard this rumor by a colleague and didn't found anything consistent in the literature.

Personally, I think that it not make much sense. Any thoughts or experiences!?


-Otto Heringer-

Sure, if the plasmid wasn't prepared properly. Dring alkaline lysis it can be nicked by the conditions, shearing of the DNA by over-vigorous shaking, vortexing or pipetting can also damage the DNA.

It is pretty uncommon though, I would still expect to see both nicked and linear forms.