Gel Bands - (Oct/27/2014 )

Hi,

I am running an experiment which gives thick gel bands on 1%agarose gels with TBE buffer which causes difficulties in determining band size.

My samples vary from 100-900ng. Right now, I am using 5ul sample and 15ul Master mix containing digestive enzymes (0.5ul each) , 2 ul suitable NEB buffer and rest water for all the samples.

Any suggestion on improving results would be welcome.I need a standazided method which can give me good results.

Thanks,

Priyanka

You might have to reduce your sample for gel run and use comb with narrow/thinner wells.