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How to reduce electrophoresis times - Impatient me needs faster results (Feb/23/2011 )

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HI all,

On so many occasions, I have come across solutions to running your gels faster/ alternatives to slab gels etc etc. and now I dont even remember the names...

Basically, I am looking for something that will reduce electrophoresis time (ET) by a huge margin. I know there are pre-cast gels that reduce the ET to 15-20 mins. There is also something else, and does not even need a transilluminator. The only problem is, I dont remember the brand names.

All suggestions are welcome. (For all of those out there like me, send words that might even be remotely similar)

Thanks :)

Ameya

-gt_ameya-

are you thinking of the e-gel system by invitrogen?

-mdfenko-

another option would be Elchrom's Origin.

And there's always the option to use Lithium borate buffer to have faster gels in normal gel chambers...

-hobglobin-

mdfenko on Wed Feb 23 15:51:20 2011 said:


are you thinking of the e-gel system by invitrogen?


Yes something like this E gel. Would you know any other products, possibly cheaper alternatives.

I remember there was this system which had crimson red gels.


Thanks for your help.

-gt_ameya-

What about Lonza:
http://www.lonza.com/group/en/products_services/products/researchproducts/flashgel.html

-adrian kohsf-

adrian kohsf on Thu Feb 24 11:04:47 2011 said:


What about Lonza:
http://www.lonza.com/group/en/products_services/products/researchproducts/flashgel.html


Adrian,

SPOT ON


Thanks!!!! :) :) :)

-gt_ameya-

How many gels do you get for $105.00 USD?

-HomeBrew-

gt_ameya on Fri Feb 25 04:54:48 2011 said:


adrian kohsf on Thu Feb 24 11:04:47 2011 said:


What about Lonza:
http://www.lonza.com/group/en/products_services/products/researchproducts/flashgel.html


Adrian,

SPOT ON


Thanks!!!! :) :) :)



LOLx... welcome.... I forget the price but I remember is expensive...

-adrian kohsf-

HomeBrew on Fri Feb 25 09:36:04 2011 said:


How many gels do you get for $105.00 USD?


I dont know.... have asked for a quote.... please dont tell me ONE. I will never get the system then...

:( + (tears in my eyes)...

-gt_ameya-

I'd appreciate it if you'd let us know... Looking over their web site, I had the sinking feeling it was one gel. The only indication I could find that it might not be was the description to the FlashGel DNA Starter Kit, which mentions it comes with "a pack of 1.2% DNA Cassettes"...

-HomeBrew-
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