PFGE gel - Can I reuse pulsed field certified gel for PFGE? (Apr/22/2009 )

I'm running Pulsed field gel electrophoresis for P.aeruginosa...
I use 1% pulsed field certified gel for seperating..

Can I reuse this gel??
And if I can reuse how many times should I reuse??

Please, advise me!!
thank you very much for your advice


-Cherman-

tana_jung on Apr 22 2009, 08:33 AM said:

I would be happy to reuse the gel for normal electrophoresis (with adjustments to the gel buffer concentration). But I would not reuse the gel for PFGE. You can't afford the same level of DNA degradation with intact chromosomes compared to plasmid DNA. It is easier to smear.

perneseblue on Apr 22 2009, 11:40 PM said:

thank you very much for your good suggestion..