Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

detection of small peptide by the SDS-PAGE gel - (May/25/2015 )


can i use gradient sds-page gel for detection of small peptide (5kDa) instead of  tris-tricine gel?

what percentage range is good for this size?

can you get me a protocol for this?



5 kDa is a little low for a good looking separation in tris-glycine.


also, what is the range of sizes you are trying to separate?


a gradient would be good for a range, but if you want to see just the 5 kDa protein then a single percentage gel of 15% should be okay (if you want use a gradient to sharpen the band then maybe 10-20% will work for you).