Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (Feb/02/2011 )
My Molecular Cloning book states that I should run the gel for five minutes at 5 V/cm before switching off and loading the samples. What do you suppose is the purpose of this initial run?
I'm guessing that its to equilibrate the gel in the buffer, but I don't know which component is doing the equilibration.