My acrylimide gel rips. - I need help keeping it frmo ripping all the time (Nov/18/2009 )
Hello, I have been doing western blotting a lot recently. When I transfer my poly acrylimide gel to the transfer setup, it always rips and folds on itself. Is their a trick to getting it off the glass panel and on to the filter paper without falling to pieces or tearing?
I remove my gel from the glass by setting the plate gel-side down in a small tub of transfer buffer and gently working the gel off the glass into the buffer. I then use a spatula to transfer the loose gel to the membrane.
Dip whatever tool you are using to move/loosen the gel into some transfer buffer before trying to get the gel off. This will stop the gel from sticking to the tool.
spellberg56 on Nov 18 2009, 02:32 PM said:
What lab rat told you is the best way to go, also you can add 10% glicerine to the gel to make more stable.