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Buffer Related Discussions
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TBE BUFFER - (reply: 2)
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OVERDILUTION OF TBE BUFFER - (reply: 1)
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Rapid western blot buffer ?? - (reply: 1)
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'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
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MgCl2 (and EDTA) in buffers - (reply: 7)
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Potassium phosphate buffer - (reply: 3)
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Making Phosphate buffer with calcium chloride - (reply: 7)
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SDS-PAGE gel and running buffer - (reply: 1)
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Purifying RNA from Solution with RNEasy? - (reply: 2)
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citrate buffer pH 8 - (reply: 1)
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Preparing Palmitate:BSA solution for treatment of cells? - (reply: 6)
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How to prepare Tris-HCl buffer for protein crystallization? What does 33mM mean? - (reply: 6)
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blocking buffer containing BSA and cross reactivity - (reply: 2)
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Reuse of SDS-PAGE electrode buffers - (reply: 2)
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Restriction buffer precipitates - (reply: 3)
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FACS Buffer - (reply: 5)
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Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
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wash solution - (reply: 1)
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restriction digestion buffers contaminated with dnase - (reply: 3)
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pH of RIPA buffer?? - (reply: 2)
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Reuse of 2-Mercaptoethanol added buffer - (reply: 1)
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Which vendor sells ready made MOPS pH7.2 solution? - (reply: 1)
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lysis buffer not lysing adherent cells? - (reply: 8)
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How to Prepare Digestion Buffer - (reply: 1)
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MES-acetate buffer recipe - (reply: 2)
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why my solution II turns cloudy during plasmid extraction? - (reply: 6)
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10 mM Na Acetate buffer preparation - (reply: 2)
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Getting rid of percipitate in Ringer solution? - (reply: 8)
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PCR buffer without Tris - (reply: 1)
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RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
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Conversion help for making a solution - (reply: 2)
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blocking and primary antibody in different buffers? - (reply: 1)
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Annexin binding buffer (urgent advice) - (reply: 2)
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help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
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RNA Buffer Extraction - (reply: 3)
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How to determine the optimal concentration of a primary antibody solution - (reply: 5)
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Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
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HEPES buffer preparation - (reply: 2)
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RBC LYSIS BUFFER - (reply: 2)
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buffer exchange - (reply: 3)
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Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
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SDS-PAGE Running Buffer contamination - (reply: 1)
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Basic general formula for making solutions? - (reply: 4)
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Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
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Stripping buffer for nitrocellulose membrane - (reply: 1)
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Can you autoclave citrate buffer? - (reply: 1)
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How to determine the volume of lisis buffer and DNA extraction buffer when the a - (reply: 5)
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SB buffer and agarose: Fragments less than 100 bp - (reply: 1)
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Co-immunoprecipitation and lysis buffers. Should I listen to my supervisor? - (reply: 2)
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PBS or TBS for washing and dilution buffer - (reply: 4)
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Effect of Sodium Azide and DTT in a buffer. - (reply: 2)
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Washing buffer and use of Protease inhibitors - (reply: 1)
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DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
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How to make up a buffer?? - (reply: 3)
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phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
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Protein purification buffer - (reply: 3)
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Storage buffer for antibodies - (reply: 3)
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Is mCherry denatured by Trypsin or RIPA buffer? - (reply: 7)
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Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
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Gooey DNA is protein samples after adding sample buffer - (reply: 1)
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protein solution cloudy after dialysis - (reply: 5)
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Triton X-100 Stock Solution - (reply: 6)
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antibiotic stock solutions - (reply: 1)
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Tissue culture treatment of cover slip - Tissue culture treatment without using amine/ thiol containing buffers (reply: 5)
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Tris glycine SDS buffer prep pH error - (reply: 2)
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ask for advice on peptide mapping - exchange buffer, visual comparision, quality criterion (reply: 7)
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NP 40 lysis buffer - (reply: 1)
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Buffer preparation - (reply: 1)
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Peptide competition assay - solvent and buffer (reply: 2)
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Bradford and triton lysis buffer - (reply: 9)
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membrane filters for chromatography buffers - (reply: 1)
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Is loading buffer ok? - (reply: 1)
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PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
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A problem when preparing 75% dioxane solution - (reply: 1)
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alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
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Citrate buffer without citric acid? - (reply: 3)
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In-Fusion (Clontech) Reaction buffer - (reply: 1)
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Removing Sodium Azide from a solution - (reply: 1)
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Removing Sodium Azide from a solution - (reply: 1)
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Calculations with x-times concentrated Buffers - (reply: 3)
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leaving gel in buffer ! - (reply: 3)
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Best method to filter a chloroform solution? - (reply: 2)
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protein phosphorylation status in sample buffer - (reply: 2)
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Gel Extraction using buffer QG then phenol/chl - Can I? (reply: 1)
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Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
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How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
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bacterial growth in buffers - (reply: 2)
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magnesium gtp solution - (reply: 2)
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Almost always NaCl in buffers - Why? - NaCl seems to be the gold standard in buffers - why? (reply: 3)
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HEPES Buffer - (reply: 3)
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Which buffer do you use for gel electrophoresis? - (reply: 9)
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FACS sheath buffer composition - (reply: 5)
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TE buffer in PEI transfection - (reply: 1)
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SDS sample buffer (loading) turns green with precipitate - strange (reply: 3)
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western blot - substarate buffer recepie (reply: 2)
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PBS/TBS buffer in ELISA - Using TBS increases the signal, why? (reply: 6)
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Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
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Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
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cathode buffer for CN-PAGE - (reply: 1)
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Proteinase K stock solution - (reply: 3)
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RIPA Buffer turns Bradford into Blue - (reply: 2)
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PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
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PCR stopped working - After changing buffer (reply: 5)
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Biotinylation of protein in tris buffer - (reply: 2)
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Detergent-based lysis buffer reciipe for E coli - (reply: 6)
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finding molar amounts in a buffer solution - (reply: 1)
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buffer calculations - (reply: 4)
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FISH (Fluorescent in situ hybridization) What volumes are recommended for buffer - Volumes for hybridization buffers and washing buffers? (reply: 2)
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HELP NEEDED: How to prepare HEPES-Tris Buffer? - (reply: 2)
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What types of buffers are in the body? - (reply: 1)
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ELISA buffer preservative - (reply: 2)
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Bacterial lysis buffer - (reply: 3)
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prepare solution - sodium hydroxide solution (reply: 2)
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Transfer buffer as running buffer? - (reply: 5)
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TAE buffer contamination - (reply: 1)
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Tris Glycine Buffer - (reply: 4)
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Molarity of Concentrated solution - (reply: 2)
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buffer ph - how to make a tris buffer (reply: 4)
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mitochondria storage - solution needed within the next 1 hr at least - long term storage (reply: 1)
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solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
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A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
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Tris buffer with EDTA - (reply: 1)
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Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
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Changing buffer from pH 7.4 to pH 5.9 - (reply: 3)
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resolving and stucking gel solutions - (reply: 2)
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Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
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potassium buffer - how to make a potassium buffer? (reply: 8)
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Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
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Which lysis buffer should I use? - (reply: 5)
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efficient stripping buffer - (reply: 2)
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How does the RBC lysis buffer work? - (reply: 3)
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Molarity of citrate phosphate buffer - (reply: 1)
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Best way to apply pvp (or a viscous solution) to coverslip - Microscopy Technique (reply: 1)
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Calcium assay: Buffer gives increase in signal, similar to agonist - (reply: 2)
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Cell Lysis Buffer - (reply: 6)
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How to make a buffer similar to PBS, pH 8,6? - (reply: 1)
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how to make Percoll solutions?! - (reply: 4)
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how does rna precipitate in LiCl solution ? - (reply: 1)
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Lysis buffer selection - (reply: 2)
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loading buffer and western blot help! - (reply: 5)
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barbital buffer???? - what for??? (reply: 1)
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Use of carbonate buffer to dilute antibody? - (reply: 4)
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Making solutions for RNA work - (reply: 1)
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column chromatography - elution buffer for proteins (reply: 2)
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Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
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EDTA addition to trypsin solution (1x) - (reply: 1)
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How to homogenise tissue in buffer? - (reply: 2)
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SB Buffer and Page Electrophoresis - (reply: 3)
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Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
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citrate buffer recipe - (reply: 2)
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organotypic brain slice cultures - modified slice buffer (reply: 1)
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Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
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autoclaving tubes and buffers (again!) - (reply: 2)
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Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
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Change of pH of Phosphate buffer with Imidazole - (reply: 1)
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PMSF solution - (reply: 3)
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MES buffer turned yellow - (reply: 5)
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buffer storage - (reply: 3)
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DTT in GST elution buffer - (reply: 1)
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Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
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Stainning of peritoneal macrophages with Oil Red O - I'm not really sure of HOW to prepare the Oil Red O solution (reply: 2)
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suitable cell lysis buffer - (reply: 1)
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Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
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protein extraction - ripa buffer vs urea buffer (reply: 1)
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Cells do not lyse in SDS buffer - (reply: 1)
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need lysis buffer for histone extraction - (reply: 1)
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PH OF BUFFERS - (reply: 1)
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lysis buffer for lymphoblast cells - (reply: 1)
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Protein extraction buffer compatible with 2D PAGE - (reply: 4)
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Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
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Sample loading buffer turning yellow. - (reply: 3)
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What's the solvent I should use to dilute my organic compounds - compounds dissolved in DMSO, but cannot dissolve in aqueous solution (reply: 4)
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Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
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DNA loading buffer - (reply: 2)
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Bromophenol blue turns yellow - during making SDS-PAGE sample buffer (reply: 1)
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What is the solution when tween80 or DMSO is not enough to dissolve plant extrac - (reply: 1)
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Cell culture medium instead of Nucleofector solution?? - (reply: 3)
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Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
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What is the solution when tween80 or DMSO is not enough to dissolve plant extrac - (reply: 3)
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What is the solution when tween80 or DMSO is not enough to dissolve plant extrac - (reply: 2)
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what is the solution when tween80 or DMSO are not enough to dissolve plant extra - (reply: 7)
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Methanol vs Isopropanol in fixing solution - (reply: 1)
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Tris acetate sds buffer - (reply: 2)
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TBE Buffer? - (reply: 4)
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how to prepare degass H2O or buffer...? - (reply: 1)
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Protein stabilization buffer needed - (reply: 1)
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Freezing Solution - (reply: 1)
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How to prepare this solution ? - (reply: 4)
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anion exchange and buffer - (reply: 1)
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How can i get this solution soluble? - Lysis buffer (reply: 2)
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T4 ligase buffer - (reply: 4)
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Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
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stability of the stripping buffer - (reply: 2)
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Help! % solution confusion - Making % stock solutions (reply: 4)
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Sodium borate buffer gel problems - (reply: 5)
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TCA precipitation from High% sucrose solutions - (reply: 1)
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Invitrogen NuPAGE Sample buffer - (reply: 1)
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Help with protein extraction buffer problem!? - (reply: 1)
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chromatin isolation buffer..where to store? - (reply: 2)
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T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
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Precipitation in DNAse I incubation buffer - (reply: 1)
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Dialysis - A question about buffers (reply: 6)
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which is the best lysis buffer for whole cell extracts? - (reply: 1)
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Blocking buffer - (reply: 7)
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Sodium borate buffer - (reply: 2)
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Qiagen RNeasy kit: Freeze homogenate in RLT buffer with B-me at -80ºC? - (reply: 3)
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Need help with GES buffer!!!! - (reply: 1)
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after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
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How to make an isotonic solution for h9c2 cells - (reply: 2)
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Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
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preparation of solutions. - had i got it right ?? (reply: 7)
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Could I raise the salt conc. in fixation solution? - (reply: 1)
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Need help creating a 1000 U/ml solution - (reply: 6)
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Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
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plasmid extraction kit wash buffer solution - (reply: 3)
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plasmid extraction kit solution composition - wash buffer (reply: 2)
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Solutions for Collection and Culture of Dust Mites - appreciate any protienal ideas (reply: 5)
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RIPA Buffer precipitation trouble - (reply: 5)
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spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
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10X MOPS buffer for RNA gels - It is yellow! (reply: 3)
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Lysis buffer - (reply: 9)
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EMSA using biotin labeled probes and buffers - (reply: 5)
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How to make digestion buffer? - (reply: 4)
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Foam in the BSA solution - (reply: 3)
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10X TBE buffer - (reply: 7)
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Incubation with washing buffer only? - (reply: 4)
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buffer for membrane protein - (reply: 1)
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Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
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HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
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Hepes-KOH buffer - (reply: 2)
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NuPAGE bis-tris gel transfer buffer question - (reply: 1)
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Why do gel electrophoresis buffers need an electrolyte? - (reply: 4)
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0.9% NaCl solution in microbiology - (reply: 2)
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5' aza-cytidine - stock solution - (reply: 4)
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Tris solution yellow?! - (reply: 3)
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pH buffer color - (reply: 2)
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Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
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immunoprecipitation lysis buffer - (reply: 6)
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sheath buffer preparation - (reply: 5)
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PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
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Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
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preparing uric acid solution - (reply: 1)
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role of NaCl in lysis buffer - (reply: 2)
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NaCl or KCl in hypotonic buffer - (reply: 1)
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dna storage buffer - (reply: 3)
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Need help desperately: Yeast tRNA and hybridization buffer - (reply: 1)
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Preparing ethidium bromide stock solution. - (reply: 2)
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LDS sample buffer in 95C - (reply: 2)
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protein in phosphate buffer precipitate out with acetonitrile - (reply: 1)
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Buffer help - Another buffer question, with a different approach (reply: 3)
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sample buffer pH - its effect?? (reply: 12)
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Restriction enzyme buffer running out ? - (reply: 1)
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Sodium Phosphate buffer creation - Need help to create this buffer (reply: 2)
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what is the glycine for in running and trans buffer？ - and what is the Tris for？ (reply: 1)
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Tris Buffer for PCR reaction - why? (reply: 1)
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Hanks balanced salt solution with or without Ca2+ and Mg2+? - (reply: 2)
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Restriction buffer composition - (reply: 2)
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Wash buffer pH - (reply: 2)
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Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
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Tris-acetate buffer? - (reply: 4)
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mitomycin c going into solution - (reply: 1)
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Protein Free Blocking Buffers - (reply: 1)
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cell lysis buffer - (reply: 1)
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Sugar solution had changed colour - strange... (reply: 8)
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Making ATP solution - (reply: 10)
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PBS and blocking buffer for ELISA applications - (reply: 1)
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0.15M Borate Buffer pH 8.3 - (reply: 3)
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Differences among Phosphate buffer, Tris-HCl and Tris Buffer - (reply: 1)
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Concentrating Protein - 8M Urea, Protein solution, concentrating (reply: 8)
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SDS PAGE: sample buffer 2-ME or DTT?? - (reply: 1)
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Tris Buffer Problem - Assignment trouble (reply: 4)
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pH of Urea and Acrylamide solutions!!! - (reply: 4)
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Tricky buffer preparation - (reply: 1)
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His tag Protein Elution - Elution Buffer (reply: 2)
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membrane protein extraction buffer - (reply: 1)
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Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
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Blocking buffer for antibodies generated in goat. - (reply: 6)
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Protein Sample Loading Buffer - (reply: 3)
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dNTP or Deoxynucleotide solution Mix - (reply: 1)
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RIPA buffer pH? - pH 8.0 or 7.6 (reply: 1)
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Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
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help~ how concentrate protein solution in large-scale - (reply: 2)
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Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
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Acid stop solution for HRP/TMB reaction - How is work? (reply: 1)
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sodium phosphate buffer recipe - (reply: 2)
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Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
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Shelf life of buffers? (unopened) - (reply: 1)
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direct lysis in Laemmli buffer - (reply: 4)
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Mannitol in Lysis Buffer - (reply: 1)
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how long will a 1M DTT solution will last in -20C - (reply: 5)
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Buffer for 5 Kb DNA fragments - (reply: 2)
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Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
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Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
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Problems with pH readings all over the place - I think the problem's in the NaCl solution (reply: 3)
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why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
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Thiamine HCl 1M solution? - can I store it at RT? (reply: 2)
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Extraction of protein from sample buffer (+DTT) - (reply: 1)
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2X sample buffer vs. 6X - (reply: 5)
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Accidentally Froze Transfer Buffer - (reply: 1)
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Protein stability in RIPA buffer at room temp - (reply: 3)
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Yellow Laemmli sample buffer - (reply: 6)
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Protein lysis buffer - (reply: 4)
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TNES buffer - TNES buffer (reply: 3)
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buffer used for making brain homogenate - (reply: 1)
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Storage of 8M Urea buffer? - I made it two weeks ago? (reply: 3)
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Will ligase buffer affect polymerase fidelity? - (reply: 2)
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Storage buffer for electrode - pH 7.0 standard buffer in stead of storage buffer? (reply: 3)
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Folic acid solution for cell culture - (reply: 2)
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Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
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Blocking buffer as antibody dilutent? - (reply: 4)
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EGTA solution storage - (reply: 1)
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Can RIPA buffer be frozen - (reply: 2)
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wash buffer for columns - how to make it? (reply: 3)
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ChIP protocol - Anyone uses solutions prepared by themselves? (reply: 2)
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Southern Blot Transfer Buffer Substitutions? - (reply: 1)
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High salt solution to precipitate RNA - (reply: 1)
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osmolality of a solution?? - (reply: 1)
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Protein in and out of solution - (reply: 1)
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western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
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What is in the Blocking solution for DIG-Southern Blot? - (reply: 2)
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PROTEIN PRECIPITATION SOLUTION - (reply: 1)
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b-mercaptoethanol in lysis buffer - (reply: 1)
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Radiation safety - Volatile components in 35S solution (reply: 1)
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Buffer Creation - Specifically acetate buffers (reply: 1)
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Lysis Buffer for Total Protein Assay - (reply: 1)
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Convert 2gl^-1 into usable unit for use in aqueous solution? - Re: CaCl2 and alkyl polyglucoside (reply: 3)
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ELISA Extraction Buffer for Plants? - (reply: 4)
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PBS not ChIP Buffer - I used PBS not ChiP dilution buffer. (reply: 8)
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Which buffer to use? - (reply: 3)
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Tissue & Cell Fixation solutions - (reply: 3)
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if antibody solution could be used again? - (reply: 8)
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Making stock solutions - (reply: 9)
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Best lysis buffer for IEF/ 2D gel? - (reply: 2)
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Quick ? about Enzyme Buffers - (reply: 3)
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freezing of digitonin solutions - (reply: 2)
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stabilization of purified protein solutions - (reply: 2)
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IEF buffer (cathod and anode) for range 4-6 - (reply: 3)
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EtBr solution - (reply: 3)
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Phospatase inhibitors needed for LDS Buffer? - (reply: 3)
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Citrate buffer preparation - extraction buffer preparation (reply: 3)
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lysis buffer to use for enzyme assay - (reply: 1)
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Problems with SDS gel - Boiling samples, autoclaved runnung buffer, 4 layers (reply: 6)
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glycine in unfolding buffer - (reply: 1)
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Glycine in unfolding buffer - (reply: 1)
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Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
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buffer - (reply: 2)