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Buffer Related Discussions
  1. Does the volume of buffer you resuspend a pellet in affect cell yield? - (reply: 1)
  2. RE: preparing a solution - (reply: 1)
  3. PVDF Membrane Floating in Reprobe Solution . . . - (reply: 5)
  4. Viral isolation kit QIA buffer substitute - (reply: 3)
  5. 0.2 M Sodium Acetate Buffer - (reply: 3)
  6. How to measure glucose concentration in a water solution? - (reply: 1)
  7. Dilution buffer - (reply: 1)
  8. PBS or TBS, where cannot use each buffers? - (reply: 1)
  9. what solution in upper layer of PCI solution? - (reply: 1)
  10. why Tris-glycine buffer divided by w/ SDS and w/o SDS? - (reply: 1)
  11. Preventing contamination in TBS/T (WB wash buffer) - (reply: 5)
  12. Stock solution of a substance in DMSO - (reply: 1)
  13. RNA extraction using Sigma lysis buffer and Qiagen spin columns - (reply: 1)
  14. Please help me determine if this math for creating a stock solution and drug tre - (reply: 2)
  15. preparing a solution - (reply: 1)
  16. RNA isolation RBC RPE buffer swap - (reply: 1)
  17. HEPES BUFFER and protein degradation - (reply: 1)
  18. Ampicillin solution does not frozen at -20 C. - (reply: 3)
  19. Sample buffer - (reply: 1)
  20. phosphate buffered saline solution for in vivo testing - (reply: 4)
  21. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
  22. Methylene Blue Solution -Scytek Laboratories - (reply: 2)
  23. Buffer in His Tag Isolation - (reply: 2)
  24. Lysis buffer preparation - (reply: 1)
  25. How to adjust the pH of a bacterial culture medium using a buffer? - (reply: 3)
  26. Transfer buffer with methanol spill - (reply: 5)
  27. TCDD (2,3,7,8-​Tetrachlorodibenzo-p-dioxin solution) cell line treatment - (reply: 1)
  28. 1 M tris pH 6.8 buffer preparation using tris and Tris HCL (No conc HCL) - (reply: 4)
  29. 5X, 6X sds gel loading buffer - (reply: 4)
  30. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
  31. solution preparation - (reply: 1)
  32. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
  33. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
  34. Friendly antibody buffer to prevent precipitation? - (reply: 3)
  35. Stock solution - (reply: 4)
  36. Zeba buffer exchange low protein yield - (reply: 6)
  37. Conversion of milligram weighing Alkaline phosphatase powder to U/L solution - (reply: 3)
  38. Kindly help in validating stock solutions calculations - (reply: 1)
  39. RNA Conditioning Solution Agilent Pico Chip - (reply: 1)
  40. Stock solution: preparation from chemicals? - (reply: 2)
  41. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
  42. Concentration of Sample Buffer to boil protein samples - (reply: 2)
  43. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
  44. Confuse on makeup of TLC solution - (reply: 4)
  45. Can I autoclave inorganic salt solutions? - (reply: 2)
  46. Three component buffer - (reply: 2)
  47. Preparing buffers for pH testing - (reply: 2)
  48. RNase-A digestion in NEB cutsmart buffer - (reply: 1)
  49. Preparing buffers for pH testing - (reply: 3)
  50. pH adjustment of 3.5% NaCl solution - (reply: 1)
  51. Transfer buffer for tris tricine gel - (reply: 12)
  52. 0.4M phosphate buffer ph 6.8 - (reply: 1)
  53. 0.1-0.25% Sodium Deoxycholate vs 420mM NaCl in TrionX-100 lysis buffer. Which is - (reply: 4)
  54. bisulfte conversion with Ampure XP beads in the solution - (reply: 2)
  55. Does TritonX-100 lysis buffer break up mitochondrial membrane? - (reply: 2)
  56. Composition of Buffer AL, Qiagen DNA minikit? - (reply: 1)
  57. Anion Exchange Buffers - (reply: 2)
  58. about sds-page buffers - (reply: 1)
  59. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
  60. Can I snap freeze my tissue (liquid nitrogen) in Lysis buffer for later RNA extr - (reply: 6)
  61. Lysis buffer for proteins - (reply: 6)
  62. Blocking buffer for biotin-labeled EMSA - (reply: 2)
  63. TE buffer pH adjustment - (reply: 1)
  64. bacterial RNA isolation from PBS buffer water - (reply: 1)
  65. solution - (reply: 7)
  66. Tissue homogenization using Potassium phosphate buffer - (reply: 1)
  67. Query regarding Preparation of salicylic acid solution for seedling treatment - (reply: 1)
  68. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  69. How to make %v/v solution using a % w/v solution - (reply: 5)
  70. Possible primer dimer problem..Need solution!!! - (reply: 2)
  71. RIPA buffer for IP and western - (reply: 1)
  72. CTab procedure is not working, results in a milky solution. - (reply: 2)
  73. Can I treat my buffer with DEPC ? - (reply: 1)
  74. How to prepare a "home-made" lysis solution compatible with RNA extracti - (reply: 1)
  75. PCR solution calculus - (reply: 4)
  76. 2x laemmli buffer - (reply: 4)
  77. Acid-Base Buffer Question - (reply: 6)
  78. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
  79. too much heating LDS-sample buffer - (reply: 3)
  80. Calculating molar ratio & dilution of protein cluster solution - (reply: 9)
  81. Glutaraldehyde solution - ok to freeze thaw or should aliquot? - (reply: 1)
  82. Effects of Freezing Point by Organic Buffers - (reply: 5)
  83. Can you store DNA in loading dye buffer short term? - (reply: 2)
  84. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  85. Heating elution buffer for plasmid prep - (reply: 1)
  86. ChIP Buffers - (reply: 1)
  87. PMSF stability in RIPA buffer - (reply: 4)
  88. Non-denaturing buffer to extract nuclear protein - (reply: 1)
  89. Any idea of the composition of the RNA Wash Buffer from Zymo Research RNA Clean - (reply: 3)
  90. 40mM EDTA in (non-metallo)protease assay buffer - (reply: 2)
  91. can buffer ever go bad? - (reply: 2)
  92. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  93. Measuring Fe(IV) concentration in solution? - (reply: 1)
  94. Mammalian Cell Lysis Buffer Triton-X100 Content - (reply: 6)
  95. Buffer Substitution: Calcium for Magnesium - (reply: 4)
  96. Sodium Acetate Buffer pH 5.2 - (reply: 3)
  97. How do I make GTE buffer for alkaline lysis? - (reply: 2)
  98. how to make fast blocking buffer in western blot? - (reply: 1)
  99. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
  100. Discard AW1, P2, P1. QC solution - (reply: 5)
  101. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
  102. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  103. Making a stock solution for my drug using DMSO - (reply: 16)
  104. 10x Laemmli buffer - (reply: 6)
  105. I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation? - (reply: 1)
  106. Semi-dry system for NuPage with MES buffer - (reply: 4)
  107. pH a Buffer - (reply: 1)
  108. Is there a buffer at acidic pH? (pH 1 and 2) - (reply: 22)
  109. TE Buffer pH 9.0 - (reply: 1)
  110. How to calculate graded umol/L concentration from stock solution - (reply: 1)
  111. Preparation of a %NACL solution - (reply: 5)
  112. Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
  113. STOCK SOLUTION - (reply: 4)
  114. Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
  115. RBC lysis buffer for Rat Blood - (reply: 1)
  116. comparing buffers? - (reply: 3)
  117. Enzymes and buffers from different companies - (reply: 6)
  118. Final concentration of prepared testing solution - (reply: 2)
  119. 5x SDS lysis buffer - (reply: 5)
  120. calculation of salts in potassium phosphate buffer - (reply: 5)
  121. EtBr in agarose gel and running buffer - (reply: 3)
  122. Lysis buffer for white matter - (reply: 1)
  123. pcr 10X buffer preparation - (reply: 2)
  124. Possible substitutions of HEPES buffer with sodium-free buffers - (reply: 2)
  125. Quantitation of protein concentration in rehydration buffer - (reply: 3)
  126. How to prepare stock solution for drugs - (reply: 1)
  127. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
  128. EDTA precipitates out of EDTA-oxalate buffer - (reply: 8)
  129. Problem with precipitating RNA from ethanol solution - (reply: 1)
  130. What is NEB's CutSmart Buffer? - (reply: 5)
  131. Buffers RNase decontamination - (reply: 1)
  132. In solution digestion - (reply: 2)
  133. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
  134. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
  135. Sodium Acetate Buffer - (reply: 10)
  136. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
  137. Making an acetate buffer - (reply: 4)
  138. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
  139. analysing an unknown solution - (reply: 10)
  140. Western blot and Triton X-100 in blocking buffer - (reply: 1)
  141. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
  142. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
  143. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  144. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
  145. Buffer fluorescence signal?! - (reply: 2)
  146. How long can I store cell lysis buffer at 4C for? - (reply: 1)
  147. Blocking solution mistake, please help! - (reply: 2)
  148. what is the contamination in buffers - (reply: 10)
  149. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  150. Best Way To Prepare Sodium Bicarbonate Solution - (reply: 1)
  151. Blocking Buffer gone bad - (reply: 1)
  152. IMAC nickel solution - (reply: 1)
  153. I forgot to put ice block in transfer buffer, does it matter? - (reply: 7)
  154. there is some way to replace MOPS in a buffer solution? - (reply: 5)
  155. Premixing gel solutions - (reply: 2)
  156. Checking pH of RNase Free Solutions - (reply: 1)
  157. How does fibronectin "crash out" of solution? - (reply: 1)
  158. Sodium Phosphate Buffer - (reply: 4)
  159. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  160. NBT solution in X-Gal staining - (reply: 1)
  161. Lysis buffer - (reply: 2)
  162. Preparing Solutions - (reply: 5)
  163. Green-dye in HRP-Conjugate solution - (reply: 7)
  164. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
  165. Anaerobic Bacteria-Cystine Sulfide Solution - (reply: 9)
  166. 5% BSA blocking buffer - (reply: 3)
  167. SB buffer recipe. - (reply: 5)
  168. solutions containing different concentrations of oxaloacetate, malate, and NADH - (reply: 1)
  169. Storing freshly autoclaved LB agar solution? - (reply: 9)
  170. Addition of sample loading buffer, before or after heating - (reply: 5)
  171. Question with lysis buffer to probe for phospho-cMet receptor - (reply: 5)
  172. Buffer in Gel - (reply: 6)
  173. TAE Vs TBE buffer - (reply: 13)
  174. Preparation of phosphate potassium buffer - (reply: 3)
  175. What is the activity of 1X Trypsin solutions? - (reply: 3)
  176. Protein precipitation from RIPA buffer to perform ELISA - (reply: 4)
  177. Making a buffer - (reply: 4)
  178. PIPES pH 8.0 in lysis buffers - (reply: 2)
  179. adjusting pH of 4x stacking buffer - (reply: 3)
  180. Store western samples in NP-40 lysis buffer? - (reply: 1)
  181. Zinc acetate buffer - (reply: 1)
  182. Tris buffer pH problem - (reply: 3)
  183. HIS elution: can imidazole be added after SDS-sample buffer? - (reply: 1)
  184. Preparing homemade XL1-blue cells in CCMB80 buffer - (reply: 1)
  185. Change in the pH of stock solution - (reply: 13)
  186. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  187. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  188. N acetyl cysteine solution for cell culture - (reply: 3)
  189. Lysis buffer for Co-IP - (reply: 2)
  190. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
  191. Secondary Ab is M.I.A. ... how long can I leave my membrane in wash buffer? - (reply: 1)
  192. Discard acrylamide solution - (reply: 3)
  193. brain tissue homogenization with sample buffer?? - (reply: 2)
  194. Buffer composition for miniprep kit - (reply: 6)
  195. Transformation Storage Buffer - too much MgSO4 - (reply: 2)
  196. 0.2M phosphate buffer for IHC - (reply: 1)
  197. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
  198. stripping buffer for western blot - (reply: 3)
  199. Nonidet P-40 (NP-40) in washing buffer after hybridisation - (reply: 2)
  200. TBE BUFFER - (reply: 2)
  201. OVERDILUTION OF TBE BUFFER - (reply: 1)
  202. Rapid western blot buffer ?? - (reply: 2)
  203. 'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
  204. MgCl2 (and EDTA) in buffers - (reply: 10)
  205. Potassium phosphate buffer - (reply: 3)
  206. Making Phosphate buffer with calcium chloride - (reply: 10)
  207. SDS-PAGE gel and running buffer - (reply: 3)
  208. Purifying RNA from Solution with RNEasy? - (reply: 2)
  209. citrate buffer pH 8 - (reply: 1)
  210. Preparing Palmitate:BSA solution for treatment of cells? - (reply: 7)
  211. How to prepare Tris-HCl buffer for protein crystallization? What does 33mM mean? - (reply: 6)
  212. blocking buffer containing BSA and cross reactivity - (reply: 2)
  213. Reuse of SDS-PAGE electrode buffers - (reply: 2)
  214. Restriction buffer precipitates - (reply: 3)
  215. FACS Buffer - (reply: 5)
  216. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
  217. wash solution - (reply: 1)
  218. restriction digestion buffers contaminated with dnase - (reply: 3)
  219. pH of RIPA buffer?? - (reply: 2)
  220. Reuse of 2-Mercaptoethanol added buffer - (reply: 1)
  221. Which vendor sells ready made MOPS pH7.2 solution? - (reply: 1)
  222. lysis buffer not lysing adherent cells? - (reply: 8)
  223. How to Prepare Digestion Buffer - (reply: 4)
  224. MES-acetate buffer recipe - (reply: 2)
  225. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  226. 10 mM Na Acetate buffer preparation - (reply: 2)
  227. Getting rid of percipitate in Ringer solution? - (reply: 8)
  228. PCR buffer without Tris - (reply: 1)
  229. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
  230. Conversion help for making a solution - (reply: 2)
  231. blocking and primary antibody in different buffers? - (reply: 1)
  232. Annexin binding buffer (urgent advice) - (reply: 2)
  233. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
  234. RNA Buffer Extraction - (reply: 3)
  235. How to determine the optimal concentration of a primary antibody solution - (reply: 5)
  236. Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
  237. HEPES buffer preparation - (reply: 2)
  238. RBC LYSIS BUFFER - (reply: 2)
  239. buffer exchange - (reply: 3)
  240. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
  241. SDS-PAGE Running Buffer contamination - (reply: 1)
  242. Basic general formula for making solutions? - (reply: 4)
  243. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
  244. Stripping buffer for nitrocellulose membrane - (reply: 1)
  245. Can you autoclave citrate buffer? - (reply: 1)
  246. How to determine the volume of lisis buffer and DNA extraction buffer when the a - (reply: 5)
  247. SB buffer and agarose: Fragments less than 100 bp - (reply: 1)
  248. Co-immunoprecipitation and lysis buffers. Should I listen to my supervisor? - (reply: 2)
  249. PBS or TBS for washing and dilution buffer - (reply: 4)
  250. Effect of Sodium Azide and DTT in a buffer. - (reply: 2)
  251. Washing buffer and use of Protease inhibitors - (reply: 1)
  252. DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
  253. How to make up a buffer?? - (reply: 3)
  254. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
  255. Protein purification buffer - (reply: 3)
  256. Storage buffer for antibodies - (reply: 3)
  257. Is mCherry denatured by Trypsin or RIPA buffer? - (reply: 7)
  258. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  259. Gooey DNA is protein samples after adding sample buffer - (reply: 1)
  260. protein solution cloudy after dialysis - (reply: 5)
  261. Triton X-100 Stock Solution - (reply: 6)
  262. antibiotic stock solutions - (reply: 1)
  263. Tissue culture treatment of cover slip - Tissue culture treatment without using amine/ thiol containing buffers (reply: 5)
  264. Tris glycine SDS buffer prep pH error - (reply: 2)
  265. ask for advice on peptide mapping - exchange buffer, visual comparision, quality criterion (reply: 7)
  266. NP 40 lysis buffer - (reply: 1)
  267. Buffer preparation - (reply: 1)
  268. Peptide competition assay - solvent and buffer (reply: 2)
  269. Bradford and triton lysis buffer - (reply: 10)
  270. membrane filters for chromatography buffers - (reply: 1)
  271. Is loading buffer ok? - (reply: 1)
  272. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  273. A problem when preparing 75% dioxane solution - (reply: 1)
  274. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  275. Citrate buffer without citric acid? - (reply: 3)
  276. In-Fusion (Clontech) Reaction buffer - (reply: 1)
  277. Removing Sodium Azide from a solution - (reply: 1)
  278. Removing Sodium Azide from a solution - (reply: 1)
  279. Calculations with x-times concentrated Buffers - (reply: 3)
  280. leaving gel in buffer ! - (reply: 3)
  281. Best method to filter a chloroform solution? - (reply: 2)
  282. protein phosphorylation status in sample buffer - (reply: 2)
  283. Gel Extraction using buffer QG then phenol/chl - Can I? (reply: 1)
  284. Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
  285. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
  286. bacterial growth in buffers - (reply: 2)
  287. magnesium gtp solution - (reply: 2)
  288. Almost always NaCl in buffers - Why? - NaCl seems to be the gold standard in buffers - why? (reply: 3)
  289. HEPES Buffer - (reply: 3)
  290. Which buffer do you use for gel electrophoresis? - (reply: 9)
  291. FACS sheath buffer composition - (reply: 5)
  292. TE buffer in PEI transfection - (reply: 1)
  293. SDS sample buffer (loading) turns green with precipitate - strange (reply: 3)
  294. western blot - substarate buffer recepie (reply: 2)
  295. PBS/TBS buffer in ELISA - Using TBS increases the signal, why? (reply: 6)
  296. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
  297. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  298. cathode buffer for CN-PAGE - (reply: 1)
  299. Proteinase K stock solution - (reply: 3)
  300. RIPA Buffer turns Bradford into Blue - (reply: 2)
  301. PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
  302. PCR stopped working - After changing buffer (reply: 5)
  303. Biotinylation of protein in tris buffer - (reply: 2)
  304. Detergent-based lysis buffer reciipe for E coli - (reply: 6)
  305. finding molar amounts in a buffer solution - (reply: 1)
  306. buffer calculations - (reply: 4)
  307. FISH (Fluorescent in situ hybridization) What volumes are recommended for buffer - Volumes for hybridization buffers and washing buffers? (reply: 2)
  308. HELP NEEDED: How to prepare HEPES-Tris Buffer? - (reply: 2)
  309. What types of buffers are in the body? - (reply: 1)
  310. ELISA buffer preservative - (reply: 2)
  311. Bacterial lysis buffer - (reply: 3)
  312. prepare solution - sodium hydroxide solution (reply: 2)
  313. Transfer buffer as running buffer? - (reply: 7)
  314. TAE buffer contamination - (reply: 1)
  315. Tris Glycine Buffer - (reply: 4)
  316. Molarity of Concentrated solution - (reply: 2)
  317. buffer ph - how to make a tris buffer (reply: 4)
  318. mitochondria storage - solution needed within the next 1 hr at least - long term storage (reply: 1)
  319. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
  320. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
  321. Tris buffer with EDTA - (reply: 1)
  322. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  323. Changing buffer from pH 7.4 to pH 5.9 - (reply: 3)
  324. resolving and stucking gel solutions - (reply: 2)
  325. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
  326. potassium buffer - how to make a potassium buffer? (reply: 8)
  327. Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
  328. Which lysis buffer should I use? - (reply: 5)
  329. efficient stripping buffer - (reply: 2)
  330. How does the RBC lysis buffer work? - (reply: 9)
  331. Molarity of citrate phosphate buffer - (reply: 1)
  332. Best way to apply pvp (or a viscous solution) to coverslip - Microscopy Technique (reply: 1)
  333. Calcium assay: Buffer gives increase in signal, similar to agonist - (reply: 2)
  334. Cell Lysis Buffer - (reply: 6)
  335. How to make a buffer similar to PBS, pH 8,6? - (reply: 1)
  336. how to make Percoll solutions?! - (reply: 8)
  337. how does rna precipitate in LiCl solution ? - (reply: 1)
  338. Lysis buffer selection - (reply: 2)
  339. loading buffer and western blot help! - (reply: 5)
  340. barbital buffer???? - what for??? (reply: 1)
  341. Use of carbonate buffer to dilute antibody? - (reply: 4)
  342. Making solutions for RNA work - (reply: 1)
  343. column chromatography - elution buffer for proteins (reply: 2)
  344. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  345. EDTA addition to trypsin solution (1x) - (reply: 1)
  346. How to homogenise tissue in buffer? - (reply: 2)
  347. SB Buffer and Page Electrophoresis - (reply: 3)
  348. Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
  349. citrate buffer recipe - (reply: 2)
  350. organotypic brain slice cultures - modified slice buffer (reply: 1)
  351. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
  352. autoclaving tubes and buffers (again!) - (reply: 2)
  353. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  354. Change of pH of Phosphate buffer with Imidazole - (reply: 1)
  355. PMSF solution - (reply: 3)
  356. MES buffer turned yellow - (reply: 5)
  357. buffer storage - (reply: 3)
  358. DTT in GST elution buffer - (reply: 1)
  359. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
  360. Stainning of peritoneal macrophages with Oil Red O - I'm not really sure of HOW to prepare the Oil Red O solution (reply: 3)
  361. suitable cell lysis buffer - (reply: 1)
  362. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
  363. protein extraction - ripa buffer vs urea buffer (reply: 1)
  364. Cells do not lyse in SDS buffer - (reply: 1)
  365. need lysis buffer for histone extraction - (reply: 1)
  366. PH OF BUFFERS - (reply: 1)
  367. lysis buffer for lymphoblast cells - (reply: 1)
  368. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
  369. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
  370. Sample loading buffer turning yellow. - (reply: 3)
  371. What's the solvent I should use to dilute my organic compounds - compounds dissolved in DMSO, but cannot dissolve in aqueous solution (reply: 4)
  372. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
  373. DNA loading buffer - (reply: 2)
  374. Bromophenol blue turns yellow - during making SDS-PAGE sample buffer (reply: 1)
  375. What is the solution when tween80 or DMSO is not enough to dissolve plant extrac - (reply: 1)
  376. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
  377. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
  378. What is the solution when tween80 or DMSO is not enough to dissolve plant extrac - (reply: 3)
  379. What is the solution when tween80 or DMSO is not enough to dissolve plant extrac - (reply: 2)
  380. what is the solution when tween80 or DMSO are not enough to dissolve plant extra - (reply: 7)
  381. Methanol vs Isopropanol in fixing solution - (reply: 1)
  382. Tris acetate sds buffer - (reply: 2)
  383. TBE Buffer? - (reply: 4)
  384. how to prepare degass H2O or buffer...? - (reply: 1)
  385. Protein stabilization buffer needed - (reply: 1)
  386. Freezing Solution - (reply: 1)
  387. How to prepare this solution ? - (reply: 4)
  388. anion exchange and buffer - (reply: 1)
  389. How can i get this solution soluble? - Lysis buffer (reply: 2)
  390. T4 ligase buffer - (reply: 4)
  391. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
  392. stability of the stripping buffer - (reply: 2)
  393. Help! % solution confusion - Making % stock solutions (reply: 4)
  394. Sodium borate buffer gel problems - (reply: 5)
  395. TCA precipitation from High% sucrose solutions - (reply: 1)
  396. Invitrogen NuPAGE Sample buffer - (reply: 1)
  397. Help with protein extraction buffer problem!? - (reply: 1)
  398. chromatin isolation buffer..where to store? - (reply: 2)
  399. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
  400. Precipitation in DNAse I incubation buffer - (reply: 1)
  401. Dialysis - A question about buffers (reply: 6)
  402. which is the best lysis buffer for whole cell extracts? - (reply: 1)
  403. Blocking buffer - (reply: 7)
  404. Sodium borate buffer - (reply: 2)
  405. Qiagen RNeasy kit: Freeze homogenate in RLT buffer with B-me at -80ºC? - (reply: 3)
  406. Need help with GES buffer!!!! - (reply: 1)
  407. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  408. How to make an isotonic solution for h9c2 cells - (reply: 2)
  409. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
  410. preparation of solutions. - had i got it right ?? (reply: 7)
  411. Could I raise the salt conc. in fixation solution? - (reply: 1)
  412. Need help creating a 1000 U/ml solution - (reply: 6)
  413. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
  414. plasmid extraction kit wash buffer solution - (reply: 3)
  415. plasmid extraction kit solution composition - wash buffer (reply: 2)
  416. Solutions for Collection and Culture of Dust Mites - appreciate any protienal ideas (reply: 5)
  417. RIPA Buffer precipitation trouble - (reply: 5)
  418. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
  419. 10X MOPS buffer for RNA gels - It is yellow! (reply: 3)
  420. Lysis buffer - (reply: 9)
  421. EMSA using biotin labeled probes and buffers - (reply: 5)
  422. How to make digestion buffer? - (reply: 4)
  423. Foam in the BSA solution - (reply: 3)
  424. 10X TBE buffer - (reply: 7)
  425. Incubation with washing buffer only? - (reply: 4)
  426. buffer for membrane protein - (reply: 1)
  427. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
  428. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
  429. Hepes-KOH buffer - (reply: 2)
  430. NuPAGE bis-tris gel transfer buffer question - (reply: 1)
  431. Why do gel electrophoresis buffers need an electrolyte? - (reply: 4)
  432. 0.9% NaCl solution in microbiology - (reply: 2)
  433. 5' aza-cytidine - stock solution - (reply: 4)
  434. Tris solution yellow?! - (reply: 3)
  435. pH buffer color - (reply: 2)
  436. Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
  437. immunoprecipitation lysis buffer - (reply: 6)
  438. sheath buffer preparation - (reply: 5)
  439. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
  440. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  441. preparing uric acid solution - (reply: 2)
  442. role of NaCl in lysis buffer - (reply: 2)
  443. NaCl or KCl in hypotonic buffer - (reply: 1)
  444. dna storage buffer - (reply: 3)
  445. Need help desperately: Yeast tRNA and hybridization buffer - (reply: 1)
  446. Preparing ethidium bromide stock solution. - (reply: 2)
  447. LDS sample buffer in 95C - (reply: 2)
  448. protein in phosphate buffer precipitate out with acetonitrile - (reply: 1)
  449. Buffer help - Another buffer question, with a different approach (reply: 3)
  450. sample buffer pH - its effect?? (reply: 12)
  451. Restriction enzyme buffer running out ? - (reply: 1)
  452. Sodium Phosphate buffer creation - Need help to create this buffer (reply: 2)
  453. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
  454. Tris Buffer for PCR reaction - why? (reply: 1)
  455. Hanks balanced salt solution with or without Ca2+ and Mg2+? - (reply: 2)
  456. Restriction buffer composition - (reply: 2)
  457. Wash buffer pH - (reply: 2)
  458. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
  459. Tris-acetate buffer? - (reply: 4)
  460. mitomycin c going into solution - (reply: 1)
  461. Protein Free Blocking Buffers - (reply: 1)
  462. cell lysis buffer - (reply: 1)
  463. Sugar solution had changed colour - strange... (reply: 8)
  464. Making ATP solution - (reply: 10)
  465. PBS and blocking buffer for ELISA applications - (reply: 1)
  466. 0.15M Borate Buffer pH 8.3 - (reply: 3)
  467. Differences among Phosphate buffer, Tris-HCl and Tris Buffer - (reply: 1)
  468. Concentrating Protein - 8M Urea, Protein solution, concentrating (reply: 8)
  469. SDS PAGE: sample buffer 2-ME or DTT?? - (reply: 1)
  470. Tris Buffer Problem - Assignment trouble (reply: 4)
  471. pH of Urea and Acrylamide solutions!!! - (reply: 4)
  472. Tricky buffer preparation - (reply: 1)
  473. His tag Protein Elution - Elution Buffer (reply: 2)
  474. membrane protein extraction buffer - (reply: 1)
  475. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
  476. Blocking buffer for antibodies generated in goat. - (reply: 6)
  477. Protein Sample Loading Buffer - (reply: 3)
  478. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  479. RIPA buffer pH? - pH 8.0 or 7.6 (reply: 1)
  480. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
  481. help~ how concentrate protein solution in large-scale - (reply: 2)
  482. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  483. Acid stop solution for HRP/TMB reaction - How is work? (reply: 1)
  484. sodium phosphate buffer recipe - (reply: 2)
  485. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
  486. Shelf life of buffers? (unopened) - (reply: 1)
  487. direct lysis in Laemmli buffer - (reply: 4)
  488. Mannitol in Lysis Buffer - (reply: 1)
  489. how long will a 1M DTT solution will last in -20C - (reply: 5)
  490. Buffer for 5 Kb DNA fragments - (reply: 2)
  491. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
  492. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
  493. Problems with pH readings all over the place - I think the problem's in the NaCl solution (reply: 3)
  494. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
  495. Thiamine HCl 1M solution? - can I store it at RT? (reply: 2)
  496. Extraction of protein from sample buffer (+DTT) - (reply: 1)
  497. 2X sample buffer vs. 6X - (reply: 5)
  498. Accidentally Froze Transfer Buffer - (reply: 1)
  499. Protein stability in RIPA buffer at room temp - (reply: 3)
  500. Yellow Laemmli sample buffer - (reply: 6)
  501. Protein lysis buffer - (reply: 4)
  502. TNES buffer - TNES buffer (reply: 3)
  503. buffer used for making brain homogenate - (reply: 1)
  504. Storage of 8M Urea buffer? - I made it two weeks ago? (reply: 3)
  505. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  506. Storage buffer for electrode - pH 7.0 standard buffer in stead of storage buffer? (reply: 3)
  507. Folic acid solution for cell culture - (reply: 2)
  508. Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
  509. Blocking buffer as antibody dilutent? - (reply: 4)
  510. EGTA solution storage - (reply: 1)
  511. Can RIPA buffer be frozen - (reply: 2)
  512. wash buffer for columns - how to make it? (reply: 3)
  513. ChIP protocol - Anyone uses solutions prepared by themselves? (reply: 2)
  514. Southern Blot Transfer Buffer Substitutions? - (reply: 1)
  515. High salt solution to precipitate RNA - (reply: 1)
  516. osmolality of a solution?? - (reply: 1)
  517. Protein in and out of solution - (reply: 1)
  518. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
  519. What is in the Blocking solution for DIG-Southern Blot? - (reply: 2)
  520. PROTEIN PRECIPITATION SOLUTION - (reply: 1)
  521. b-mercaptoethanol in lysis buffer - (reply: 1)
  522. Radiation safety - Volatile components in 35S solution (reply: 1)
  523. Buffer Creation - Specifically acetate buffers (reply: 1)
  524. Lysis Buffer for Total Protein Assay - (reply: 1)
  525. Convert 2gl^-1 into usable unit for use in aqueous solution? - Re: CaCl2 and alkyl polyglucoside (reply: 3)
  526. ELISA Extraction Buffer for Plants? - (reply: 4)
  527. PBS not ChIP Buffer - I used PBS not ChiP dilution buffer. (reply: 8)
  528. Which buffer to use? - (reply: 3)
  529. Tissue & Cell Fixation solutions - (reply: 3)
  530. if antibody solution could be used again? - (reply: 8)
  531. Making stock solutions - (reply: 9)
  532. Best lysis buffer for IEF/ 2D gel? - (reply: 2)
  533. Quick ? about Enzyme Buffers - (reply: 3)
  534. freezing of digitonin solutions - (reply: 2)
  535. stabilization of purified protein solutions - (reply: 2)
  536. IEF buffer (cathod and anode) for range 4-6 - (reply: 3)
  537. EtBr solution - (reply: 3)
  538. Phospatase inhibitors needed for LDS Buffer? - (reply: 3)
  539. Citrate buffer preparation - extraction buffer preparation (reply: 3)
  540. lysis buffer to use for enzyme assay - (reply: 1)
  541. Problems with SDS gel - Boiling samples, autoclaved runnung buffer, 4 layers (reply: 6)
  542. glycine in unfolding buffer - (reply: 1)
  543. Glycine in unfolding buffer - (reply: 1)
  544. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
  545. buffer - (reply: 2)