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Stripping buffer for nitrocellulose membrane - (Nov/18/2011 )

Can some body please tell me the receipe for making stripping buffer which can be used on nitrocellulose membrane? Thanks a lot.


-Gaurav S. Choudhary-

I use this recipe and strip my nitrocellulose blot for 30 min at 56­ oC with shaking.

100mM β-mercaptoethanol
2% SDS
62.5 mM Tris (pH 6.7)