How to make up a buffer?? - (Sep/27/2011 )

Can any one please help explain the calculations on how to make up the following;
lysis buffer (50mM Tris-Cl pH 8, 10mM EDTA, 2% SDS).
I'm totally lost on where to begin.

So what do you know about making up buffers?

If starting from powdered chemicals, you will need the following equations V=n/C (where V =volume, n=number of moles and C=concentration) and n=m/M_r (where n= number of moles, m= mass and M_r= molar mass).

Please read the Material safety data sheet (MSDS) before using any chemicals, and use appropriate safety equipment - if you don't have them, ASK FOR THEM!

EDTA is a bit tricky - you need the correct pH before it will dissolve.

Only thing i know about making buffers is using Henderson Hasselback equation pH= pKa + log ( / Im guessing the 2% SDS is made up like this- 2g of SDS dissolved in 100ml of dH20.
I'm not even sure if thats correct and if its to be made up to a volume of 100ml. I'm really lost for this question no matter how hard i try i can't come up with a logic answer.

Haras911 on Wed Oct 5 13:05:08 2011 said: