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Excess bromophenol blue in 2-D rehydration buffer - (May/10/2012 )

if i add excess of bromophenol blue into the sample that is to be used for rehydrtaing the IPG strip (which i actually have done by mistake) what will be its effect on IEF run. guys please help me out its urgent


it will not have an effect on the run itself (it may have a visual effect during the run).