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Excess bromophenol blue in 2-D rehydration buffer - (May/10/2012 )

if i add excess of bromophenol blue into the sample that is to be used for rehydrtaing the IPG strip (which i actually have done by mistake) what will be its effect on IEF run. guys please help me out its urgent

-sudamit-

it will not have an effect on the run itself (it may have a visual effect during the run).

-mdfenko-