plasmid extraction kit wash buffer solution - (Mar/08/2010 )
Hi
unfortunately I have lost my plasmid extraction kit solutions and now I have unused columns, I have the recipe for resuspension, lysis and neutralization solutions but I dont know the composition of wash buffer, does any body know how can I make it myself?
regards
-sara.r-
sara.r on Mar 8 2010, 07:29 PM said:
Hi
unfortunately I have lost my plasmid extraction kit solutions and now I have unused columns, I have the recipe for resuspension, lysis and neutralization solutions but I dont know the composition of wash buffer, does any body know how can I make it myself?
regards
unfortunately I have lost my plasmid extraction kit solutions and now I have unused columns, I have the recipe for resuspension, lysis and neutralization solutions but I dont know the composition of wash buffer, does any body know how can I make it myself?
regards
The easiest way is trying the washing solution of other purification kit. I usually don't distinguish the one for the plasmid extraction kit and the one for the gel extraction kit, they are similar.
-Quasimondo-
I would says these washbuffers are just 70% ethanol.
-tea-test-
tea-test on Mar 8 2010, 10:33 AM said:
I would says these washbuffers are just 70% ethanol.
You can check the Buffer composition here: http://openwetware.org/wiki/Miniprep/Qiagen_kit
-snoopyx-