need lysis buffer for histone extraction - (Jun/30/2010 )

Hello all,
Can anyone tell me how to extract histones from cells for western blot? or is their any lysis buffer especially used for histones extraction?
thanking you..

deep on Jul 1 2010, 02:37 AM said:

I ued some antiboides against H3 and some histone modifications, and the lysis buffer I used was quite standard. You can use NP-40 I guess or do the lysis directly in sds 1x