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need lysis buffer for histone extraction - (Jun/30/2010 )

Hello all,
Can anyone tell me how to extract histones from cells for western blot? or is their any lysis buffer especially used for histones extraction?
thanking you..

-deep-

deep on Jul 1 2010, 02:37 AM said:

Hello all,
Can anyone tell me how to extract histones from cells for western blot? or is their any lysis buffer especially used for histones extraction?
thanking you..


I ued some antiboides against H3 and some histone modifications, and the lysis buffer I used was quite standard. You can use NP-40 I guess or do the lysis directly in sds 1x

-laurequillo-