how to make fast blocking buffer in western blot? - (Nov/30/2013 )
Recently, I saw some fast blocking buffer products named superblock or rapid block, which can finish blocking PVDF in 10 mins. Is there somebody know how to prepare fast blocking buffer ?
There's nothing magical about them, most blocking solutions will actually do something similar - something like 90% of the blocking is done in the first 10 minutes of incubation with any protein solution. 1/2 an hour should be close to 100%