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Tris buffer with EDTA - (Dec/16/2010 )

I have to make Tris buffer with EDTA of pH 8.0. I am little confused with either to add the EDTA before adjusting the pH or first adjust the pH and then put the but the EDTA.
I shall be very thankful to you for your kind reply.


You can adjust after adding the EDTA. If you are using Tris-base, the EDTA will lower the pH. You can make adjustments after adding the EDTA.

-lab rat-