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RBC lysis buffer for Rat Blood - (Jul/16/2013 )

Hi everyone,

Long time no see!

Can anyone recommend a good RBC lysis buffer for rat blood?

I have tried the Biolegend buffer (ammonium chloride, potassium carbonate, and EDTA) and it just doesn't work well compared to when I use mouse blood (I have tried different times etc).

I also tried the DAKO Utilyse kit (which is FANTASTIC for mouse blood) but it didn't work at all for my rat blood.

Any suggestions would be greatly appreciated

Clare

-Clare-

Maybe you can try to use ddH2O for 20-30s

-xiaogu985-