MES buffer turned yellow - (Aug/03/2010 )
Hi all!
Does anyone knows if I can still use NuPAGEŽ MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!
try it on unimportant samples.
but, in general, when in doubt, throw it out.
I did a western blot a few weeks ago with NuPage MES that turned yellow, and I had no issues. Invitrogen also said it shouldn't cause any problems...light exposure in the lab can also cause it to turn yellow over time.
purdue_biomed on Tue Aug 3 15:08:30 2010 said:
I did a western blot a few weeks ago with NuPage MES that turned yellow, and I had no issues. Invitrogen also said it shouldn't cause any problems...light exposure in the lab can also cause it to turn yellow over time.
Thanks a lot! NuPage buffers are rather expensive to through away when in doubt.
yeah u can always give it a shot before deciding against it but i wud also second wat md says... if no other optins available.. and u in doubt never use it!!! (tat is wat he meant by throwing away!!! i guess 
)
and yeah if things are expensive take better care!!! 
good luck!!
katenkak on Tue Aug 3 08:44:23 2010 said:
Hi all!
Does anyone knows if I can still use NuPAGE® MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!
LOL... you had it turned yellow...
I just got mine and is already in yellow.... duh....
Yes, I agree that this buffer is not cheap.