Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Proteinase K stock solution - (Mar/14/2011 )


I want to prepare a stock solution of proteinase K. Since my powder is not DNAse-free, I want to inactivate any contaminating DNAse without affecting proteinase K. How can I do this?



proteinase k is fully active in 0.5% sds.


And it is active at high concentrations of EDTA. Adding EDTA will chelate the Mg++ ions, a necessary co-factor to DNAse.


Ok, thanks.