What is NEB's CutSmart Buffer? - (Mar/26/2013 )

Not really sure if this belongs here. It just seems like this CutSmart buffer appeared over night. Is this a replacement for the EcoRI buffer? Has anyone used it? It is reporting 100% activity in a double digest that NEB previously showed incompatible (my previous reactions). Can anyone confirm this?

-jerryshelly1-

Jerry, I'm not sure what the CutSmart buffer is made of, but "super buffers" can be very effective and have been around for a long time. An example is Mike McClelland's old KGB (Potassium Glutamate) buffer: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC334647/?page=1

-ff11-

That is interesting. I am glad that info is not proprietary. I am going to try this in some of my non-compatible enzyme digestions. Thanks for the link.

-jerryshelly1-

It's buffer 4 with BSA added, I suspect

-Ahrenhase-

Exactly. The composition is on their web site, and it is buffer 4 + BSA.

-phage434-

Ahh. I should have done more research. Thanks everyone. I guess it could be slightly beneficial.

-jerryshelly1-