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Effect of Sodium Azide and DTT in a buffer. - (Oct/19/2011 )


Can anyone please tell me the role of Sodium Azide and DTT in a buffer solution. I am asking it in relevance with biotin-streptavidin and dig-antidig construct formation in DNA.


-Saurabh Raj-

The sodium azide acts as an antimicrobial agent to eliminate growth of bacteria. The DTT is a reducing agent, reducing unwanted oxidation of your reactants.


Thanks a lot :-)

-Saurabh Raj-