Effect of Sodium Azide and DTT in a buffer. - (Oct/19/2011 )
Can anyone please tell me the role of Sodium Azide and DTT in a buffer solution. I am asking it in relevance with biotin-streptavidin and dig-antidig construct formation in DNA.
The sodium azide acts as an antimicrobial agent to eliminate growth of bacteria. The DTT is a reducing agent, reducing unwanted oxidation of your reactants.
Thanks a lot :-)