Green-dye in HRP-Conjugate solution - (Oct/26/2012 )

Does anyone know which is the green-dye in HRP-Conjugate ready-to-use solution in ELISA kits?

Which company?

for example from abcam elisa kit

Probably TMB tetramethylbenzidine having looked at a product description on the Abcam website...

TMB is the chromogen that changes colour in the presence of substrate and HRP.

The green dye is simply there as a visual to aid in the loading of wells. You could do some detective work and match the absorption spectrum with common green dyes if your spec scans.

Ben Lomond on Wed Oct 31 02:42:14 2012 said:

TMB is blue/greenish, depending on pH, isn't it?

Indeed. The green dye is simply there as a visual to aid the loading of HRP-Conjugate solution. The green dye (probably food dye) must not affect HRP function.

Sotirios on Wed Oct 31 08:54:53 2012 said:

Ah, I see what you mean - added before the substrate. Nice idea.