PIPES pH 8.0 in lysis buffers - (Sep/01/2012 )
I wonder why some biologists use PIPES pH 8.0 in lysis buffers in Protocols?
whereas the buffering range of it is 6.1-7.5 pH.
You can find those protocols even in high rank universities.
buffers are routinely misused when the procedure works anyway (e.g. tris used for stacking gel buffers in sds-page).
however, sometimes buffers are blended to extend buffering range and/or to buffer a pH that would otherwise have to be reached with a incompatible buffer salt.
are there other buffer salts in your lysis media?
Cell Lysis buffer
5 mM PIPES pH 8.0
85 mM KCL
I have sent an Email to Dr. Farnham, and I did not received reply till now.