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Can I treat my buffer with DEPC ? - (Sep/08/2014 )

So I prepared a buffer for native protein extraction:

 

25 mM Tris

100 mM NaCl

10% glicerol

 

Can I treat this buffer with DEPC ?

 

 

-Juanhijuan-

No, Tris will hydrolyze DEPC. You can use PBS buffer instead.

-tkf-