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Kindly help in validating stock solutions calculations - (Jan/05/2016 )

Hello everyone,


As title suggests, kindly assist me in validating attached stock solution calculations, are they correct? 




Thanks and Warm Regards,


Attached File


Hi Adnan.


Barring differences in molecular weight from different sources, the Tris-Cl calculations are correct.


Under your Tris-Cl/Tween, you say you are adding 0.1% Tween-20. Rather, you are supposed to add pure tween, the amounts you have specified are correct. The final, not the initial, concentration v/v is 0.1% (0.1 mL Tween-20 / 100 mL solution).


The 1N NaOH is correct, except under 0.1N NaOH, volume water should be 900 uL.





-Michael Starr-