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how does rna precipitate in LiCl solution ? - (Oct/23/2010 )

HI all

I have been isolating rna manually from leaves of arabidopsis plants using phenol/sds method and using licl as additional step for precipitation of rna. I had to precipitate the rna in 2M Licl in 4C but i did it at -20C for overnight . I wonder how rna precipiates in -20C when the solution became ice.

Please help.


Lithium and other cations help to "shield" or neutralize negative charges on RNA/DNA. Time and temperature probably only effect yield. o/n precipitation at either temperature probably isn't necessary. More discussion here:

-David C H-