# 2X sample buffer vs. 6X - (Jul/20/2009 )

What is the difference between 2X vs. 6X sample buffer and how do you know how much you should add of each into your samples before loading your gel? Thanks!

-Princesslinda-

THe difference just lies in the concentration.

2X means you need to do a 2X dilution. So say.. you wanna load 10ul, that will be 10ul/2 = 5ul of your sample buffer to load to 5ul of your samples.

6X means a 6X dilution is required, so say... for 10ul, it will be 10ul/6 = 1.7ul of your sample buffer to load to 8.3ul of your samples.

-jiajia1987-

so for example, if I have a stock that has a concentration of 10 micrograms/microliter and I want a concentration per lane of 100 microgram/microliter...I would need 10 microliters to get this concentration. How much 6X would I need to add to this?

-Princesslinda-

Add 2 ul to 10 ul. It's close enough -- you need not worry that your loading dye is not precisely 1x, just that it is sufficient to serve its purpose -- to weigh down the DNA so it won't float out, and give you an observable marker for the run.

-HomeBrew-

The difference is 4x .

-mikew-

It means how concentrated it is, ie how many times (hence the X) working concentration (or 1X) it is.
So, for example, your 2X buffer is 2 times more concentrated than a working concentration of the buffer.

PS.

The difference is 4x smile
hahaha good one mikew!!

-leelee-