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Store western samples in NP-40 lysis buffer? - (Aug/10/2012 )

Hello,

I was just curious if I could store my western samples in NP-40 lysis buffer? Usually I just add loading dye and Beta - Mercap and boil it, but I am going to be short on time tomorrow and was wondering if I could just freeze it down and add the reducing reagent later?

Thanks,
Wes

-Druss00-

Yes, -20 will be fine for a few weeks, longer than that and -80 is best. You can add the reducing agent and loading buffer and boil then freeze if you prefer.

-bob1-