Store western samples in NP-40 lysis buffer? - (Aug/11/2012 )
I was just curious if I could store my western samples in NP-40 lysis buffer? Usually I just add loading dye and Beta - Mercap and boil it, but I am going to be short on time tomorrow and was wondering if I could just freeze it down and add the reducing reagent later?
Yes, -20 will be fine for a few weeks, longer than that and -80 is best. You can add the reducing agent and loading buffer and boil then freeze if you prefer.