Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

Methanol vs Isopropanol in fixing solution - (Jun/10/2010 )

General question: What is the difference between using methanol/acetic acid versus isopropanol/acetic acid when fixing proteins in gels?

-polaris-

functionally, no difference.

concentration of alcohol necessary is different (i think you need 10% isopropanol as opposed to 30-50% methanol).

-mdfenko-