OVERDILUTION OF TBE BUFFER - (May/01/2012 )
I wanted to make a 1x TBE solution from a 1 Litre bottle of Lonza Accugene 10x TBE buffer. However, instead of adding 9 Litres of milliq water to the buffer I added 10-11 litres of milliq water.
I wanted to know two things
can i use this TBE as it is to make DNA gels for genotyping
could i just get another bottle of 10x TBE add it to my existing stock then bring up to 20 litres with milliq to even out my error
It will probably work as is. You should make your gels with the same buffer.
You can fix this as you suggest by making additional buffer, if you would like.