Protocol Online logo
Top : New Forum Archives (2009-): : General Lab Techniques

OVERDILUTION OF TBE BUFFER - (May/01/2012 )

HI ALL

I wanted to make a 1x TBE solution from a 1 Litre bottle of Lonza Accugene 10x TBE buffer. However, instead of adding 9 Litres of milliq water to the buffer I added 10-11 litres of milliq water.

I wanted to know two things

can i use this TBE as it is to make DNA gels for genotyping

or

could i just get another bottle of 10x TBE add it to my existing stock then bring up to 20 litres with milliq to even out my error


thanks

-helencollins560-

It will probably work as is. You should make your gels with the same buffer.

You can fix this as you suggest by making additional buffer, if you would like.

-phage434-