buffer exchange - (Dec/11/2011 )
So, I have a purified protein, and I have to exchange the buffer (DTT interfering with downstream binding to the Ni column).
The problem is that I have very small volume (10 uL, 6 uM) of the protein?
Could anyone recommend a type of column to use?
Anyone has experience with low volumes?
Thanks a lot!!
you could use a sephadex g-25 spin column but you will significantly dilute your protein.
you can try drop dialysis.
I thought that drop dialysis is used for nucleic acids. At least I use it for that only.
I will try sephadex g 25 column. But I'm still worried about the small volume.. :/
Actually I'm going to order this:
They say it works with very small volumes.
Does anyone have experaience with it?