Any idea of the composition of the RNA Wash Buffer from Zymo Research RNA Clean - (Mar/17/2014 )
does anybody has any idea concerning the composition of the RNA Wash Buffer from Zymo Research RNA Clean & Concentrator kit? It contains EtOH (80%), so I guess whether I can simply use 80% EtOH as "backup" when the buffer is almost finished.
all the best,
A guess -- it would be buffered at pH 7.5 (or perhaps lower -- you could measure it) likely with Tris. Make sure it is RNAse free.
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Yes, but 80% ethanol made with pH 8 Tris-EDTA buffer.