Any idea of the composition of the RNA Wash Buffer from Zymo Research RNA Clean - (Mar/17/2014 )

Dear all,

does anybody has any idea concerning the composition of the RNA Wash Buffer from Zymo Research RNA Clean & Concentrator kit? It contains EtOH (80%), so I guess whether I can simply use 80% EtOH as "backup" when the buffer is almost finished.

Thanks,

all the best,

Mark

A guess -- it would be buffered at pH 7.5 (or perhaps lower -- you could measure it) likely with Tris. Make sure it is RNAse free.

Hi guys,

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Feel free to let me know if you need anything in your lab. We will be glad to provide free samples.

Lulu: lulu@earthox.net

Thanks,

Lulu

Yes, but 80% ethanol made with pH 8 Tris-EDTA buffer.