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wash buffer for columns - how to make it? (Jun/04/2009 )

Hello,

were running out of wash buffer for spin columns. The manufacturer seems not to be able to provide some extra stuff.

Any ideas how to make the buffer myself? It is the buffer to which you always have to add ethanol before use. I guess its pretty much the same for any spin column kit. Thanks! Cycle

-cycle-

The Qiagen PE wash buffer is:

* 10 mM Tris-HCl pH 7.5
* 80% ethanol

See http://openwetware.org/wiki/Miniprep/Qiagen_kit

-phage434-

THANKS! Ill try and tell what happens,

cycle

-cycle-

This is a seriously great link!

-jiajia1987-