Does TritonX-100 lysis buffer break up mitochondrial membrane? - (May/09/2015 )
TritonX-100 Lysis buffer recipe = 25 mM Tris•HCl pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 5% glycerol
I am sure it won't break up nuclear membrane but not sure whether it can break up mitochondria membrane.
Thank you very much
-asaren-
yes it does (personal experience)
sodium deoxycholate is used for nuclear membrane.
-Curtis-
Curtis on Sat May 9 17:04:58 2015 said:
yes it does (personal experience)
sodium deoxycholate is used for nuclear membrane.
Thank Curtis. I am confused about this for some time, because some of the protocols (eg. ABCAM) states that mild lysis buffer like tritonX-100 won't lyse/break mitochondrial membrane. SDS seems to be the one to break mitochondria in RIPA buffer.
-asaren-