pH of RIPA buffer?? - (Feb/21/2012 )
I have prepared RIPA buffer with the following ingredients:
50mM TRIS, pH8
0.5% sod. deoxycholate
Immediately after buffer preparation I measured its pH and it was 8.35.
I would like to know should I re-adjust the pH back to 8?
one more thing, regarding storage, some members recommend that it should be at -20, but others say that as long as there is no protease or phosphatase inhibitor cocktails it can be stored at refregirator at 4, which is the best temperature for storage?
To be safer i think you can adjust the pH to 8. regarding the storage i always store at 4 degrees that is RIPA without protease and phosphatase inhibitors. i would recommend to add the inhibitors freshly during lysis. hope this helps...
if you are determining the pH after adding sds then i wouldn't worry about it.
sds makes pH readings questionable (it precipitates in and blocks the porous plug on the electrode due to kcl).