western blot stripping buffer - please recomend a stripping buffer for re-bloting (May/04/2009 )
Could anyone recommend a stripping buffer for re-bloting western blot membranes? Thanks.
I use the following:
100mM 2-mercapto ethanol
62.5 mM Tris HCl PH6.7
If it is PVDF membrane i use 0.2 N NaoH.
Its so convinient and time also less.