Secondary Ab is M.I.A. ... how long can I leave my membrane in wash buffer? - (Jun/27/2012 )
So one of my lab mates used the last of the secondary I planned to use today without replacing it. I started rinsing my membranes before checking to make sure I had the antibody I needed, rookie mistake. Is it okay to leave the membranes in my wash buffer (same as the antibody buffer I use) at 4 degC for an extra day or so? Any advice would be appreciated!
The membranes will be fine in the wash buffer, but any antibody you have on there will get progressively washed off so you may have to re-do the primary. You could leave in PBS or TBS which would be better than a buffer with detergent.