Tris acetate sds buffer - (Jun/09/2010 )
Does anyone know how to make Tris-acetate running buffer for SDS-PAGE ?
I use this http://products.invitrogen.com/ivgn/produc...=Search-Product
But I wanted to make it myself. Anyone do this?
Here's the recipe (see here):
NuPAGEŽ Tris-Acetate SDS Running Buffer
The NuPAGEŽ Tris-Acetate SDS Running Buffer (20X) is available from Invitrogen
50 mM Tricine
50 mM Tris base
1. To prepare 500 ml of 20 X NuPAGEŽ Tris-Acetate SDS Running Buffer, dissolve the following reagents to 400 ml ultrapure water:
Tricine 89.5 g
Tris Base 60.6 g
SDS 10.0 g
2. Mix well and adjust the volume to 500 ml with ultrapure water.
3. Store at +4° C. The buffer is stable for 6 months when stored at +4° C.
4. For electrophoresis, dilute this buffer to 1X with water. The pH of the 1X solution is 8.24. Do not use acid or base to adjust the pH.
wow. Thanks thats great, didn't think invitrogen would do this. thanks.