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lysis buffer to use for enzyme assay - (Feb/22/2009 )


i'd like to do an enzyme activity assay comparing treated/untreated cells. is there a particular buffer i should use to lyse the cells but not denature the proteins?

thank you in advance



I use 50 mM Tris pH8 with 10 % glycerol or 100 mM phosphate pH 7.2 with 10 % glycerol. Also, u may have to add other things like ATP, CoASH or NAD(P)H if yr enzyme needs it.



-T C-