# Making ATP solution - (Nov/11/2009 )

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Help...........
I am trying to make a 10mls of ATP 10Mm solution the MW is 605.2

My balance is in grams and I am struggling...
any help would be good.

-Tamarind-

Having problems making a stable 10ml 10Mm solution.
MW is 605.2 my scales are in grams
Help.....

-Tamarind-

10 mmol/l means 6.05g/l
balance in grams then 10 ml is impossible

-Gerard-

10 mM (note the case, not Mm) is 1/100 mole per liter, so you would need 605/100 = 6.05 grams/liter to make a 10 mM solution. You need 10 ml, or 1/100 of a liter, so you need .0605 g or 60.5 mg of ATP in 10 ml for your solution. I would not make it this way, but would instead make a more concentrated solution and dilute it. For example, you could make a 1 M solution with 0.605 grams in 1 ml. Then you could make the 10 mM solution by diluting 100x. Since you want 10 ml, you put 100 ul of 1 M ATP soltution in 9.9 ml of water. You need to think about the stability of your high concentration stock solution -- in this case, freezing at -20 will be stable for a few months, but you would want to avoid too many freeze/thaw cycles.

-phage434-

Gerard on Nov 11 2009, 06:54 AM said:

10 mmol/l means 6.05g/l
balance in grams then 10 ml is impossible

So I need to make litre?

-Tamarind-

Can't i take it down to 0.06052g to get 10mls?

-Tamarind-

phage434 on Nov 11 2009, 07:30 AM said:

10 mM (note the case, not Mm) is 1/100 mole per liter, so you would need 605/100 = 6.05 grams/liter to make a 10 mM solution. You need 10 ml, or 1/100 of a liter, so you need .0605 g or 60.5 mg of ATP in 10 ml for your solution. I would not make it this way, but would instead make a more concentrated solution and dilute it. For example, you could make a 1 M solution with 0.605 grams in 1 ml. Then you could make the 10 mM solution by diluting 100x. Since you want 10 ml, you put 100 ul of 1 M ATP soltution in 9.9 ml of water. You need to think about the stability of your high concentration stock solution -- in this case, freezing at -20 will be stable for a few months, but you would want to avoid too many freeze/thaw cycles.

Thank you sooooooooooooo much, I will try this in a moment. Sorry about the case, was gettin desperate.

-Tamarind-

I tried the 0.605g in 1ml.
ATP does not dissolve properly.
I did dilute down from there and have frozen in 2ml aliquots. will see what happens when I try the samples.

I resorted to the other method using 0.0605g in 10mls ,
then aliquot into 2mls and freeze.
Again will see what I get.

Thanks for the help

-Tamarind-

Tamarind on Nov 11 2009, 11:40 AM said:

I tried the 0.605g in 1ml.
ATP does not dissolve properly.
I did dilute down from there and have frozen in 2ml aliquots. will see what happens when I try the samples.

I resorted to the other method using 0.0605g in 10mls ,
then aliquot into 2mls and freeze.
Again will see what I get.

Thanks for the help

you have to adjust the pH to ~7 or higher to get it all in solution at 1M (we adjust to 7).

-mdfenko-

mdfenko on Nov 13 2009, 10:20 PM said:

Tamarind on Nov 11 2009, 11:40 AM said:

I tried the 0.605g in 1ml.
ATP does not dissolve properly.
I did dilute down from there and have frozen in 2ml aliquots. will see what happens when I try the samples.

I resorted to the other method using 0.0605g in 10mls ,
then aliquot into 2mls and freeze.
Again will see what I get.

Thanks for the help

you have to adjust the pH to ~7 or higher to get it all in solution at 1M (we adjust to 7).

-arc1-
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